| A comparative study on acetyl-CoA synthesising enzymes in spinal cord from cows, horses and pigs. | |
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MedLine Citation:
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PMID: 2572376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Comparative data are presented of the activities of pyruvate dehydrogenase complex and acetyl-CoA synthetase and of the acetate content in homogenates from ventral grey matter in spinal cord from cows and two non-ruminant species, pigs and horses. The methods used in the study are evaluated and discussed. 2. The total pyruvate dehydrogenase complex activity was 24.9-29.9 mU/mg protein and did not differ between the species. The part of the complex that was in active form at the sampling occasion was 60, 85 and 95% in cows, pigs and horses, respectively. 3. Acetyl-CoA synthetase activity differed significantly between the species and was 0.93, 1.28 and 2.61 mU/mg protein in pigs, cows and horses, respectively. The highest cytosolic activity was found in the horses. Acetate concentration at half maximal reaction velocity (at saturating CoA and ATP levels) was found to be 0.15-0.70 mM and did not differ between the species. 4. Acetate content was 63, 83 and 96 micrograms/g wet wt in cows, horses and pigs, respectively. 5. It is concluded that there seems to be no striking difference in acetyl-CoA synthesis in peripheral nerves between ruminants and non-ruminant species. |
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Authors:
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C Björkman |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Volume: 93 ISSN: 0742-8413 ISO Abbreviation: Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol. Publication Date: 1989 |
Date Detail:
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Created Date: 1989-12-19 Completed Date: 1989-12-19 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 8310013 Medline TA: Comp Biochem Physiol C Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 201-6 Citation Subset: IM |
Affiliation:
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Swedish University of Agricultural Sciences, Department of Cattle and Sheep Diseases, Uppsala. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetate-CoA Ligase
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metabolism Acetates / analysis Acetyl Coenzyme A / biosynthesis* Animals Cattle / metabolism* Female Horses / metabolism* Male Pyruvate Dehydrogenase Complex / metabolism Spinal Cord / enzymology* Swine / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Pyruvate Dehydrogenase Complex; 72-89-9/Acetyl Coenzyme A; EC 6.2.1.1/Acetate-CoA Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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