Document Detail


A comparative molecular analysis of water-filled limestone sinkholes in north-eastern Mexico.
MedLine Citation:
PMID:  20738374     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Sistema Zacatón in north-eastern Mexico is host to several deep, water-filled, anoxic, karstic sinkholes (cenotes). These cenotes were explored, mapped, and geochemically and microbiologically sampled by the autonomous underwater vehicle deep phreatic thermal explorer (DEPTHX). The community structure of the filterable fraction of the water column and extensive microbial mats that coat the cenote walls was investigated by comparative analysis of small-subunit (SSU) 16S rRNA gene sequences. Full-length Sanger gene sequence analysis revealed novel microbial diversity that included three putative bacterial candidate phyla and three additional groups that showed high intra-clade distance with poorly characterized bacterial candidate phyla. Limited functional gene sequence analysis in these anoxic environments identified genes associated with methanogenesis, sulfate reduction and anaerobic ammonium oxidation. A directed, barcoded amplicon, multiplex pyrosequencing approach was employed to compare ∼100 000 bacterial SSU gene sequences from water column and wall microbial mat samples from five cenotes in Sistema Zacatón. A new, high-resolution sequence distribution profile (SDP) method identified changes in specific phylogenetic types (phylotypes) in microbial mats at varied depths; Mantel tests showed a correlation of the genetic distances between mat communities in two cenotes and the geographic location of each cenote. Community structure profiles from the water column of three neighbouring cenotes showed distinct variation; statistically significant differences in the concentration of geochemical constituents suggest that the variation observed in microbial communities between neighbouring cenotes are due to geochemical variation.
Authors:
Jason W Sahl; Marcus O Gary; J Kirk Harris; John R Spear
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Publication Detail:
Type:  Journal Article     Date:  2010-08-25
Journal Detail:
Title:  Environmental microbiology     Volume:  13     ISSN:  1462-2920     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  226-40     Citation Subset:  IM    
Copyright Information:
© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
Affiliation:
Environmental Science and Engineering, Colorado School of Mines, Golden, CO 80401, USA. Department of geological sciences, University of Texas, Austin, TX, USA. Pediatrics, University of Colorado, Denver, CO 80045, USA.
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