Document Detail


A comparative evaluation of three washing procedures for minimizing exogenous trace element contamination in fetal scalp hair of various obstetric outcomes.
MedLine Citation:
PMID:  18286236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparative evaluation of three washing methods for removing exogenous elemental contamination from fetal scalp hair is described. A total of 12 samples, including 6 from normal pregnancies and 6 from abruptio placentae (AP) cases, were used. Each was divided into four subsamples, with one left unwashed for comparison, while three were subjected to one of the three washing methods. The first consisted of agitating hair for 5 min in sequential and equal measures (30 ml) of acetone and water, followed by rinsing with acetone, and the process repeated twice. In the second and third, hair was agitated for 30 min in aqueous solutions of sodium lauryl sulfate (0.5%, w/v), and Triton X-100 (0.5%, v/v), respectively, followed by sequential rinsing with deionized water and acetone. After drying and microwave-assisted digestion in concentrated nitric acid, the subsamples' concentrations of zinc, copper, cadmium, lead, mercury and selenium were determined using inductively coupled mass spectrometry. All washed subsamples, irrespective of washing method used, had lower elemental concentrations than the corresponding, unwashed subsamples. These differences, taken as presumptive indicators of exogenous contamination, showed only small variations (<5%) between the three washing methods, irrespective of element, mode of delivery, and obstetric outcome. However, the contamination levels elicited by each of the three methods varied in magnitude according to individual elements, with those of copper being highest, followed by those of zinc, cadmium, mercury, selenium, and lead. They also varied according to obstetric outcome, being highest in the AP subsamples, while in the normal pregnancy group, copper and zinc contamination levels in the subsamples from neonates delivered by elective caesarean section were higher than in those from normal-delivery neonates. The data from this study suggest that the three washing methods used have comparable efficacies in removing exogenous elemental contamination from fetal hair, further inferring that it is probably a simple biological matrix to decontaminate. Possible contributory factors are discussed and contrasted with those affecting adult hair.
Authors:
Ibrahim B-A Razagui
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-02-20
Journal Detail:
Title:  Biological trace element research     Volume:  123     ISSN:  0163-4984     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-22     Completed Date:  2008-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  47-57     Citation Subset:  IM    
Affiliation:
Academic Department of Obstetrics and Gynaecology, University of Hull Postgraduate Medical Institute, Hull, UK. ibarazagui@aol.com
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MeSH Terms
Descriptor/Qualifier:
Female
Fetus / chemistry*
Humans
Irrigation / methods*
Mass Spectrometry
Pregnancy
Pregnancy Outcome*
Trace Elements / isolation & purification*
Chemical
Reg. No./Substance:
0/Trace Elements

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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