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A comparative evaluation of prebiotic oligosaccharides using in vitro cultures of infant fecal microbiome.
MedLine Citation:
PMID:  25239906     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objective of this study was to systematically assess the bifidogenic effect of three commonly used prebiotic products using in vitro cultures of infant fecal samples. Fresh stool samples collected from six term infants each exclusively fed human milk (n=3) or infant formula (n=3) at 28 days of age were used as inocula. The following prebiotic products were added at concentrations applicable to infant formula: Vivinal® GOS 15 (containing 28.5% galacto-oligosaccharide, referred to as GOS) at 7.2g/L, Beneo® HP (99.5% long-chain inulin, IN) at 0.8g/L, Beneo® Synergy 1 (enriched oligofructose and inulin, OF-IN) at 4g/L, and a combination of Vivinal® GOS 15 (7.2g/L) and Beneo® HP (0.8g/L) (GOS-IN). The growth of total bacteria, Bifidobacterium, Bacteroides, B. longum, and E. coli was quantified using specific qPCR. Bifidobacterium was also enumerated on selective Beerens agar plates, with representative colonies identified by sequencing their 16S rRNA genes. Volatile fatty acids (VFA) and pH in the cultures were also determined. Irrespective of the feeding methods, the GOS product, either alone or in combination with Beneo® HP, resulted in substantially higher growth of total bifidobacteria, and much of this growth was attributed to growth of B. longum. The Beneo® Synergy 1 also increased the abundance of total bifidobacteria and B. longum. Corresponding to the increases in these two bacterial groups, acetic acid concentrations were higher, while there was a trend of lower E. coli levels and pH. The lower pH and higher acetic acid might be directly responsible for the lower E. coli population. At the concentrations studied, the GOS product was more bifidogenic and potent in inhibiting E. coli than the other products tested. These results suggest that supplementation of infant formula with GOS may increase intestinal bifidobacteria and benefit infant health.
Authors:
J Stiverson; T Williams; J Chen; S Adams; D Hustead; P Price; J Guerrieri; J Deacon; Z Yu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-19
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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