Document Detail

A comparative evaluation of accelerated solvent extraction and Polytron extraction for quantification of lipids and extractable organochlorine in fish.
MedLine Citation:
PMID:  14581049     Owner:  NLM     Status:  MEDLINE    
Optimal conditions for extraction methods using an accelerated solvent extractor (ASE) and a Polytron homogenizer were established for quantification of (crude) lipids and extractable organochlorine (EOCl) in fish. The two methods, an ASE consecutive extraction at 55 and 100 degrees C for ground, freeze-dried filets, and a Polytron extraction at room temperature with four repetitions for ground, wet filets and with two repetitions for wet fish livers were evaluated in terms of reproducibility and comparability. With respect to lipid measurement, the two methods have comparable reproducibility and the experimental errors are relatively small compared to natural variations of fish to fish within a sampling site. For EOCl measurement, the relative standard deviation tends to increase with decreasing levels of EOCl in specimens. An important factor for the relative standard deviation of measurements of analytes present at trace levels in fish matrices is sampling error in taking aliquots. Statistical analysis using a paired comparison design showed that measurement values (crude lipids or EOCl) obtained from these two extraction methods are, in general, not equivalent. There is a good correlation between the two methods for lipid measurement; whereas for EOCl measurements, high degrees of correlation exist within the exposed fish group, but not in the reference group.
Wenshan Zhuang; Bruce McKague; Douglas Reeve; John Carey
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemosphere     Volume:  54     ISSN:  0045-6535     ISO Abbreviation:  Chemosphere     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-10-28     Completed Date:  2004-03-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  England    
Other Details:
Languages:  eng     Pagination:  467-80     Citation Subset:  IM    
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College St., Toronto, ON, Canada M5S 3E5.
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MeSH Terms
Chemistry Techniques, Analytical / methods*
Environmental Monitoring / methods
Evaluation Studies as Topic
Hydrocarbons, Chlorinated / isolation & purification*
Lipids / analysis*
Liver / chemistry
Reference Values
Reproducibility of Results
Reg. No./Substance:
0/Hydrocarbons, Chlorinated; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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