| A comparative analysis of the zona pellucida birefringence of fresh and frozen-thawed human embryos. | |
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MedLine Citation:
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PMID: 19736257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cryopreservation of human embryos is thought to induce alteration in the glycoprotein matrix and lead to zona change. However, this assumption has been full of controversies till now. The objective of this study was to evaluate the effect of cryopreservation on zona pellucida of human embryos. Fresh (n=106, from 40 patients) and frozen-thawed embryos (n=123, from 40 patients) were obtained from consenting patients who received conventional IVF and ICSI treatment. The birefringence of zona pellucida in human fresh and frozen-thawed embryos was imaged and quantitatively analyzed using polarized light microscopy before embryo transfer. There was no significant difference in retardance and thickness of the zona pellucida multilaminar structure between the two groups. Pregnancy and implantation rates of transferred fresh and frozen-thawed embryos were also compared. No significant difference was found in the rates of clinical pregnancy (47.5 vs 37.5%) and implantation (24.5 vs 23.2%) between the two groups. This study suggests that there is no significant change in the zona pellucida birefringence of human embryos before and after cryopreservation. |
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Authors:
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Yi-Fan Gu; Chang-Fu Lu; Ge Lin; Guang-Xiu Lu |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: 139 ISSN: 1741-7899 ISO Abbreviation: Reproduction Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-17 Completed Date: 2010-03-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
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Languages: eng Pagination: 121-7 Citation Subset: IM |
Affiliation:
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Institute of Reproductive and Stem Cell Engineering, Central South University, Xiangya Road 84, Changsha 410078, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Birefringence* Cryopreservation* Embryo Implantation Embryo, Mammalian* / cytology Female Fertilization in Vitro / methods Humans Microscopy, Polarization Pregnancy Pregnancy Rate Reproducibility of Results Zona Pellucida / chemistry* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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