Document Detail


A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells.
MedLine Citation:
PMID:  18318837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). To identify the genes responsible for hepatic differentiation, we examined the gene expression profiles of AT-MSC-derived hepatocytes (AT-MSC-Hepa) using several microarray methods. The resulting sets of differentially expressed genes (1639 clones) were comprehensively analyzed to identify the pathways expressed in AT-MSC-Hepa. Clustering analysis revealed a striking similarity of gene clusters between AT-MSC-Hepa and the whole liver, indicating that AT-MSC-Hepa were similar to liver with regard to gene expression. Further analysis showed that enriched categories of genes and signaling pathways such as complementary activation and the blood clotting cascade in the AT-MSC-Hepa were relevant to liver-specific functions. Notably, decreases in Twist and Snail expression indicated that mesenchymal-to-epithelial transition occurred in the differentiation of AT-MSCs into hepatocytes. Our data show a similarity between AT-MSC-Hepa and the liver, suggesting that AT-MSCs are modulated by their environmental conditions, and that AT-MSC-Hepa may be useful in basic studies of liver function as well as in the development of stem cell-based therapy.
Authors:
Yusuke Yamamoto; Agnieszka Banas; Shigenori Murata; Madoka Ishikawa; Chun R Lim; Takumi Teratani; Izuho Hatada; Kenichi Matsubara; Takashi Kato; Takahiro Ochiya
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The FEBS journal     Volume:  275     ISSN:  1742-464X     ISO Abbreviation:  FEBS J.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-05     Completed Date:  2008-05-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101229646     Medline TA:  FEBS J     Country:  England    
Other Details:
Languages:  eng     Pagination:  1260-73     Citation Subset:  IM    
Affiliation:
Section for Studies on Metastasis, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / cytology*,  metabolism
Adult Stem Cells / cytology,  metabolism
Cell Differentiation / genetics*
Gene Expression Profiling*
Hepatocytes / cytology,  metabolism
Humans
Liver / cytology*,  metabolism
Mesenchymal Stem Cells / cytology*,  metabolism
Oligonucleotide Array Sequence Analysis
Signal Transduction*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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