| A common element involved in transcriptional regulation of two DNA alkylation repair genes (MAG and MGT1) of Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 8246943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Saccharomyces cerevisiae MAG gene encodes a 3-methyladenine DNA glycosylase that protects cells from killing by alkylating agents. MAG mRNA levels are induced not only by alkylating agents but also by DNA-damaging agents that do not produce alkylated DNA. We constructed a MAG-lacZ gene fusion to help identify the cis-acting promoter elements involved in regulating MAG expression. Deletion analysis defined the presence of one upstream activating sequence and one upstream repressing sequence (URS) and suggested the presence of a second URS. One of the MAG URS elements matches a decamer consensus sequence present in the promoters of 11 other S. cerevisiae DNA repair and metabolism genes, including the MGT1 gene, which encodes an O6-methylguanine DNA repair methyltransferase. Two proteins of 26 and 39 kDa bind specifically to the MAG and MGT1 URS elements. We suggest that the URS-binding proteins may play an important role in the coordinate regulation of these S. cerevisiae DNA repair genes. |
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Authors:
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W Xiao; K K Singh; B Chen; L Samson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 13 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-01-03 Completed Date: 1994-01-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7213-21 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, Massachusetts 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkylation Base Sequence Cloning, Molecular Consensus Sequence DNA Damage / genetics DNA Repair / genetics* DNA, Fungal / genetics, metabolism Gene Expression Regulation, Fungal Genes, Fungal* Genes, Regulator Lac Operon Molecular Sequence Data Promoter Regions, Genetic Protein Binding Saccharomyces cerevisiae / genetics*, metabolism Sequence Deletion Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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CA55042/CA/NCI NIH HHS; ES03926/ES/NIEHS NIH HHS; IF 32 CA 59271-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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