Document Detail


A commercial pregnancy test modified for field studies of fetal loss.
MedLine Citation:
PMID:  9564555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe simple modifications to the ICON II hCG (URINE) pregnancy test to provide a sensitive and specific urinary assay for hCG in field studies of fetal loss. The modified assay had a qualitative lower limit of detection of 0.30 IU/l, a 50% qualitative limit of 0.61 IU/l, a 100% qualitative limit of 1.16 IU/l, and a quantitative limit of 0.80 IU/l. Coefficients of variation ranged from 9.9% to 21.1%. Parallelism was observed among serially diluted subject samples. We used the assay in an 11-month prospective study of fetal loss in rural Bangladesh in which urine samples were collected twice-weekly from 494 women; 330 pregnancies and 93 fetal losses were detected. The median time to a positive pregnancy diagnosis was day 26 from last menses. The modified assay provided qualitative detection of early pregnancy comparable to laboratory assays, and appears to be well suited for use in epidemiologic or rural-population fetal loss studies.
Authors:
D J Holman; F N Rasheed; C M Stroud; E Brindle; K A O'Connor; K L Campbell
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  271     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-06-04     Completed Date:  1998-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-44     Citation Subset:  IM    
Affiliation:
Department of Anthropology, Pennsylvania State University, University Park 16802, USA. holman@pop.psu.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Analysis of Variance
Bangladesh
Chorionic Gonadotropin / urine*
Female
Fetal Death / epidemiology*
Humans
Immunoassay / methods
Menstruation / physiology
Middle Aged
Pregnancy
Pregnancy Tests, Immunologic / methods*
Reagent Kits, Diagnostic
Reference Standards
Sensitivity and Specificity
Time Factors
Grant Support
ID/Acronym/Agency:
1HD28263-01/HD/NICHD NIH HHS; F32HD07994-02/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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