| The comet assay to determine the mode of cell death for the ultrasonic delivery of doxorubicin to human leukemia (HL-60 Cells) from Pluronic P105 micelles. | |
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MedLine Citation:
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PMID: 16292892 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm(2) ultrasound in the presence of 1% Pluronic P105 and 1.67 microg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis. |
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Authors:
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Ghaleb A Husseini; Kim L O'Neill; William G Pitt |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Technology in cancer research & treatment Volume: 4 ISSN: 1533-0346 ISO Abbreviation: Technol. Cancer Res. Treat. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-18 Completed Date: 2006-02-23 Revised Date: 2013-02-21 |
Medline Journal Info:
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Nlm Unique ID: 101140941 Medline TA: Technol Cancer Res Treat Country: United States |
Other Details:
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Languages: eng Pagination: 707-11 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, American University of Sharjah, United Arab Emirates. ghusseini@ausharjah.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibiotics, Antineoplastic
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administration & dosage* Apoptosis / drug effects* Comet Assay* DNA Damage DNA, Neoplasm / drug effects Dose-Response Relationship, Drug Doxorubicin / administration & dosage* Drug Delivery Systems HL-60 Cells / drug effects Humans Micelles Poloxamer / administration & dosage* Ultrasonic Therapy* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA098138/CA/NCI NIH HHS; R01 CA098138-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/DNA, Neoplasm; 0/Micelles; 106392-12-5/Poloxamer; 23214-92-8/Doxorubicin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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