Document Detail

The comet assay to determine the mode of cell death for the ultrasonic delivery of doxorubicin to human leukemia (HL-60 Cells) from Pluronic P105 micelles.
MedLine Citation:
PMID:  16292892     Owner:  NLM     Status:  MEDLINE    
This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm(2) ultrasound in the presence of 1% Pluronic P105 and 1.67 microg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis.
Ghaleb A Husseini; Kim L O'Neill; William G Pitt
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Technology in cancer research & treatment     Volume:  4     ISSN:  1533-0346     ISO Abbreviation:  Technol. Cancer Res. Treat.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-18     Completed Date:  2006-02-23     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  101140941     Medline TA:  Technol Cancer Res Treat     Country:  United States    
Other Details:
Languages:  eng     Pagination:  707-11     Citation Subset:  IM    
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MeSH Terms
Antibiotics, Antineoplastic / administration & dosage*
Apoptosis / drug effects*
Comet Assay*
DNA Damage
DNA, Neoplasm / drug effects
Dose-Response Relationship, Drug
Doxorubicin / administration & dosage*
Drug Delivery Systems
HL-60 Cells / drug effects
Poloxamer / administration & dosage*
Ultrasonic Therapy*
Grant Support
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/DNA, Neoplasm; 0/Micelles; 106392-12-5/Poloxamer; 80168379AG/Doxorubicin

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