| A combined study of behavior and Fos expression in limbic structures after re-testing Wistar rats in the elevated plus-maze. | |
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MedLine Citation:
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PMID: 20100550 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The elevated plus-maze is an animal model used to study anxiety. In a second session, rats show a reduction in the exploratory behavior even when the two sessions are separated by intervals as large as 7 days. The aim of the present study was to investigate whether the reduction in the exploratory behavior is maintained after intervals larger than 7 days. Additionally, we aimed at investigating eventual correlations between behaviors in the plus-maze and activation of limbic structures as measured by Fos protein expression after the second session. Rats were tested for 5 min in the elevated plus-maze and re-tested 3, 9 or 33 days later. Other groups were tested only once. The rat brains were processed for immunohistochemical detection of Fos protein. The results show a decrease in the open arms exploration in the second trial with intervals of 3, 9 and 33 days. The expression of Fos protein in the piriform cortex, septal nucleus and paraventricular hypothalamic nucleus in the groups tested with intervals of 9 and 33 days were statistically different from the other groups. The alterations observed in exploratory behavior in the second session in the plus-maze did not correlate with Fos expression. In conclusion, although the specific test conditions were sufficient to evoke behavioral alterations in exploration in the elevated plus-maze, they were enough to induce significant Fos protein expression in piriform cortex, septal nucleus and thalamic and hypothalamic paraventricular nuclei but not in other areas such as dorsomedial nucleus of the hypothalamus and amygdala nuclei, known to be also active participants in circuits controlling fear and anxiety. |
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Authors:
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O Y Galvis-Alonso; A M B Garcia; M J Orejarena; M R Lamprea; S Botelho; C A Conde; S Morato; N Garcia-Cairasco |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-25 |
Journal Detail:
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Title: Brain research bulletin Volume: 81 ISSN: 1873-2747 ISO Abbreviation: Brain Res. Bull. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-06-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605818 Medline TA: Brain Res Bull Country: United States |
Other Details:
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Languages: eng Pagination: 595-9 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Neurophysiology and Experimental Neuroethology Laboratory - FMRP - USP, 14049-900 Ribeirao Preto, Sao Paulo, Brazil. ngcairas@fmrp.usp.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Cell Count Cell Nucleus / metabolism Cerebral Cortex / metabolism Exploratory Behavior / physiology* Immunohistochemistry Limbic System / metabolism* Male Maze Learning / physiology* Motor Activity / physiology Neurons / metabolism Paraventricular Hypothalamic Nucleus / metabolism Proto-Oncogene Proteins c-fos / metabolism* Psychomotor Performance / physiology Rats Rats, Wistar Septal Nuclei / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-fos |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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