| A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms. | |
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MedLine Citation:
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PMID: 17258338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest. |
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Authors:
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Rudolf Gmür; Helga Lüthi-Schaller |
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Publication Detail:
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Type: Journal Article Date: 2006-12-28 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 69 ISSN: 0167-7012 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-16 Completed Date: 2007-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 402-5 Citation Subset: IM |
Affiliation:
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Institute of Oral Biology, Section of Oral Microbiology and General Immunology, University of Zürich, Plattenstrasse 11, 8032 Zürich, Switzerland. gmuer@zzmk.unizh.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
/
genetics,
isolation & purification* Dental Plaque / microbiology* Fluorescent Antibody Technique, Direct / methods* Humans In Situ Hybridization, Fluorescence / methods* Microscopy, Fluorescence Periodontitis / microbiology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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