| comK prophage junction fragments as markers for Listeria monocytogenes genotypes unique to individual meat and poultry processing plants and a model for rapid niche-specific adaptation, biofilm formation, and persistence. | |
| | |
MedLine Citation:
|
PMID: 21441318 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Different strains of Listeria monocytogenes are well known to persist in individual food processing plants and to contaminate foods for many years; however, the specific genotypic and phenotypic mechanisms responsible for persistence of these unique strains remain largely unknown. Based on sequences in comK prophage junction fragments, different strains of epidemic clones (ECs), which included ECII, ECIII, and ECV, were identified and shown to be specific to individual meat and poultry processing plants. The comK prophage-containing strains showed significantly higher cell densities after incubation at 30°C for 48 h on meat and poultry food-conditioning films than did strains lacking the comK prophage (P < 0.05). Overall, the type of strain, the type of conditioning film, and the interaction between the two were all highly significant (P < 0.001). Recombination analysis indicated that the comK prophage junction fragments in these strains had evolved due to extensive recombination. Based on the results of the present study, we propose a novel model in which the concept of defective comK prophage was replaced with the rapid adaptation island (RAI). Genes within the RAI were recharacterized as "adaptons," as these genes may allow L. monocytogenes to rapidly adapt to different food processing facilities and foods. If confirmed, the model presented would help explain Listeria's rapid niche adaptation, biofilm formation, persistence, and subsequent transmission to foods. Also, comK prophage junction fragment sequences may permit accurate tracking of persistent strains back to and within individual food processing operations and thus allow the design of more effective intervention strategies to reduce contamination and enhance food safety. |
| | |
Authors:
|
Bindhu Verghese; Mei Lok; Jia Wen; Valentina Alessandria; Yi Chen; Sophia Kathariou; Stephen Knabel |
Related Documents
:
|
21805308 - Characterization of indigenous chicken production systems in kenya. 21073258 - Extraction and analysis of polyphenols: recent trends. 21167618 - Survival of brucella spp. in mineral water, milk and yogurt. 21347428 - Division of labor associated with brood rearing in the honey bee: how does it translate... 2333838 - Food coloring and monosodium glutamate: effects on the cephalic phase of gastric acid s... 3165178 - Changes in mandibular position during treatment with disk-repositioning onlays: a roent... |
Publication Detail:
|
Type: Journal Article Date: 2011-03-25 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-05-12 Completed Date: 2011-08-22 Revised Date: 2013-05-24 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 3279-92 Citation Subset: IM |
Affiliation:
|
Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA. sjk9@psu.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/JF791254; JF791255; JF791256; JF791257; JF791258; JF791259; JF791260; JF791261; JF791262; JF791263; JF791264; JF791265; JF791266; JF791267; JF791268; JF791269; JF791270; JF791271; JF791272; JF791273; JF791274; JF791275; JF791276; JF791277; JF791278; JF791279; JF791280; JF791281; JF791282; JF791283; JF791284; JF791285; JF791286; JF791287; JF791288; JF791289; JF791290; JF791291; JF791292; JF791293; JF791294; JF791295; JF791296; JF791297; JF791298; JF791299; JF791300; JF791301; JF791302; JF791303; JF791304; JF791305; JF791306; JF791307; JF791308; JF791309; JF791310; JF791311; JF791312; JF791313; JF791314; JF791315; JF791316; JF791317; JF791318; JF791319 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacterial Typing Techniques* Biofilms / growth & development DNA, Bacterial / chemistry, genetics Environmental Microbiology* Evolution, Molecular Food-Processing Industry Genetic Variation* Genotype Listeria monocytogenes / classification, genetics*, isolation & purification, virology* Meat / microbiology* Molecular Epidemiology Molecular Sequence Data Prophages / genetics* Recombination, Genetic Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial |
| Comments/Corrections | |
Erratum In:
|
Appl Environ Microbiol. 2011 Jul;77(14):5064 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sensitive detection of Shiga Toxin 2 and some of its variants in environmental samples by a novel im...
Next Document: Novel Bacillus thuringiensis ?-endotoxin active against Locusta migratoria manilensis.