Document Detail

comK prophage junction fragments as markers for Listeria monocytogenes genotypes unique to individual meat and poultry processing plants and a model for rapid niche-specific adaptation, biofilm formation, and persistence.
MedLine Citation:
PMID:  21441318     Owner:  NLM     Status:  MEDLINE    
Different strains of Listeria monocytogenes are well known to persist in individual food processing plants and to contaminate foods for many years; however, the specific genotypic and phenotypic mechanisms responsible for persistence of these unique strains remain largely unknown. Based on sequences in comK prophage junction fragments, different strains of epidemic clones (ECs), which included ECII, ECIII, and ECV, were identified and shown to be specific to individual meat and poultry processing plants. The comK prophage-containing strains showed significantly higher cell densities after incubation at 30°C for 48 h on meat and poultry food-conditioning films than did strains lacking the comK prophage (P < 0.05). Overall, the type of strain, the type of conditioning film, and the interaction between the two were all highly significant (P < 0.001). Recombination analysis indicated that the comK prophage junction fragments in these strains had evolved due to extensive recombination. Based on the results of the present study, we propose a novel model in which the concept of defective comK prophage was replaced with the rapid adaptation island (RAI). Genes within the RAI were recharacterized as "adaptons," as these genes may allow L. monocytogenes to rapidly adapt to different food processing facilities and foods. If confirmed, the model presented would help explain Listeria's rapid niche adaptation, biofilm formation, persistence, and subsequent transmission to foods. Also, comK prophage junction fragment sequences may permit accurate tracking of persistent strains back to and within individual food processing operations and thus allow the design of more effective intervention strategies to reduce contamination and enhance food safety.
Bindhu Verghese; Mei Lok; Jia Wen; Valentina Alessandria; Yi Chen; Sophia Kathariou; Stephen Knabel
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Publication Detail:
Type:  Journal Article     Date:  2011-03-25
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-12     Completed Date:  2011-08-22     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3279-92     Citation Subset:  IM    
Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JF791254;  JF791255;  JF791256;  JF791257;  JF791258;  JF791259;  JF791260;  JF791261;  JF791262;  JF791263;  JF791264;  JF791265;  JF791266;  JF791267;  JF791268;  JF791269;  JF791270;  JF791271;  JF791272;  JF791273;  JF791274;  JF791275;  JF791276;  JF791277;  JF791278;  JF791279;  JF791280;  JF791281;  JF791282;  JF791283;  JF791284;  JF791285;  JF791286;  JF791287;  JF791288;  JF791289;  JF791290;  JF791291;  JF791292;  JF791293;  JF791294;  JF791295;  JF791296;  JF791297;  JF791298;  JF791299;  JF791300;  JF791301;  JF791302;  JF791303;  JF791304;  JF791305;  JF791306;  JF791307;  JF791308;  JF791309;  JF791310;  JF791311;  JF791312;  JF791313;  JF791314;  JF791315;  JF791316;  JF791317;  JF791318;  JF791319
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MeSH Terms
Bacterial Typing Techniques*
Biofilms / growth & development
DNA, Bacterial / chemistry,  genetics
Environmental Microbiology*
Evolution, Molecular
Food-Processing Industry
Genetic Variation*
Listeria monocytogenes / classification,  genetics*,  isolation & purification,  virology*
Meat / microbiology*
Molecular Epidemiology
Molecular Sequence Data
Prophages / genetics*
Recombination, Genetic
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA, Bacterial
Erratum In:
Appl Environ Microbiol. 2011 Jul;77(14):5064

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