Document Detail


comK prophage junction fragments as markers for Listeria monocytogenes genotypes unique to individual meat and poultry processing plants and a model for rapid niche-specific adaptation, biofilm formation, and persistence.
MedLine Citation:
PMID:  21441318     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Different strains of Listeria monocytogenes are well known to persist in individual food processing plants and to contaminate foods for many years; however, the specific genotypic and phenotypic mechanisms responsible for persistence of these unique strains remain largely unknown. Based on sequences in comK prophage junction fragments, different strains of epidemic clones (ECs), which included ECII, ECIII, and ECV, were identified and shown to be specific to individual meat and poultry processing plants. The comK prophage-containing strains showed significantly higher cell densities after incubation at 30°C for 48 h on meat and poultry food-conditioning films than did strains lacking the comK prophage (P < 0.05). Overall, the type of strain, the type of conditioning film, and the interaction between the two were all highly significant (P < 0.001). Recombination analysis indicated that the comK prophage junction fragments in these strains had evolved due to extensive recombination. Based on the results of the present study, we propose a novel model in which the concept of defective comK prophage was replaced with the rapid adaptation island (RAI). Genes within the RAI were recharacterized as "adaptons," as these genes may allow L. monocytogenes to rapidly adapt to different food processing facilities and foods. If confirmed, the model presented would help explain Listeria's rapid niche adaptation, biofilm formation, persistence, and subsequent transmission to foods. Also, comK prophage junction fragment sequences may permit accurate tracking of persistent strains back to and within individual food processing operations and thus allow the design of more effective intervention strategies to reduce contamination and enhance food safety.
Authors:
Bindhu Verghese; Mei Lok; Jia Wen; Valentina Alessandria; Yi Chen; Sophia Kathariou; Stephen Knabel
Related Documents :
21805308 - Characterization of indigenous chicken production systems in kenya.
24551828 - Determination of 4-methylimidazole and 2-acetyl-4()- tetrahydroxybutylimidazole in cara...
21073258 - Extraction and analysis of polyphenols: recent trends.
23755758 - Detection of bovine alpha-s1-casein in term and preterm human colostrum with proteomic ...
2333838 - Food coloring and monosodium glutamate: effects on the cephalic phase of gastric acid s...
3165178 - Changes in mandibular position during treatment with disk-repositioning onlays: a roent...
Publication Detail:
Type:  Journal Article     Date:  2011-03-25
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-12     Completed Date:  2011-08-22     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3279-92     Citation Subset:  IM    
Affiliation:
Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA. sjk9@psu.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JF791254;  JF791255;  JF791256;  JF791257;  JF791258;  JF791259;  JF791260;  JF791261;  JF791262;  JF791263;  JF791264;  JF791265;  JF791266;  JF791267;  JF791268;  JF791269;  JF791270;  JF791271;  JF791272;  JF791273;  JF791274;  JF791275;  JF791276;  JF791277;  JF791278;  JF791279;  JF791280;  JF791281;  JF791282;  JF791283;  JF791284;  JF791285;  JF791286;  JF791287;  JF791288;  JF791289;  JF791290;  JF791291;  JF791292;  JF791293;  JF791294;  JF791295;  JF791296;  JF791297;  JF791298;  JF791299;  JF791300;  JF791301;  JF791302;  JF791303;  JF791304;  JF791305;  JF791306;  JF791307;  JF791308;  JF791309;  JF791310;  JF791311;  JF791312;  JF791313;  JF791314;  JF791315;  JF791316;  JF791317;  JF791318;  JF791319
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Typing Techniques*
Biofilms / growth & development
DNA, Bacterial / chemistry,  genetics
Environmental Microbiology*
Evolution, Molecular
Food-Processing Industry
Genetic Variation*
Genotype
Listeria monocytogenes / classification,  genetics*,  isolation & purification,  virology*
Meat / microbiology*
Molecular Epidemiology
Molecular Sequence Data
Prophages / genetics*
Recombination, Genetic
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections
Erratum In:
Appl Environ Microbiol. 2011 Jul;77(14):5064

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sensitive detection of Shiga Toxin 2 and some of its variants in environmental samples by a novel im...
Next Document:  Novel Bacillus thuringiensis ?-endotoxin active against Locusta migratoria manilensis.