Document Detail


On-column derivatization and analysis of amino acids, peptides, and alkylamines by anhydrides using capillary electrophoresis.
MedLine Citation:
PMID:  11001229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work demonstrates the use of an in-capillary procedure for derivatization of amino acids, peptides, and alkylamines by anhydrides using capillary electrophoresis (CE). Migrating in an uncoated fused-silica capillary, plugs of substrate and anhydride are injected separately and electrophoresed. Differential transport velocities permit the separate zones to penetrate each other under an applied field, thereby facilitating reaction. In initial experiments the extent of reaction between tryptophan and acetic anhydride was examined and product amounts quantitated by CE. In separate experiments a series of amino acids and peptides were injected into the capillary and reacted with phthalic anhydride on-column to yield the phthalic derivatized species. Finally, on-column derivatization of alkylamines with phthalic anhydride was investigated and electrophoretic mobility related to molecular weight of the derivatized amines. These procedures illustrate the use of the capillary as a microreactor in the facile synthesis of derivatized molecules and ease of quantitation of reaction products under conditions of electrophoresis.
Authors:
Y Zhang; F A Gomez
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Electrophoresis     Volume:  21     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-18     Completed Date:  2001-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  3305-10     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, California State University, Los Angeles 90032-8202, USA.
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MeSH Terms
Descriptor/Qualifier:
Amines / analysis*
Amino Acids / analysis*
Anhydrides
Electrophoresis, Capillary / instrumentation,  methods
Equipment Design
Indicators and Reagents
Peptides / analysis*
Chemical
Reg. No./Substance:
0/Amines; 0/Amino Acids; 0/Anhydrides; 0/Indicators and Reagents; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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