Document Detail


A collaborative study to evaluate qualitatively powdered baby food validation samples artificially contaminated with Salmonella anatum.
MedLine Citation:
PMID:  15527917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nineteen laboratories participated in a collaborative study to evaluate the performance of validation samples consisting of powdered infant formula and cereal-based weaning food artificially contaminated with low levels of Salmonella anatum. The Standard method BS EN ISO 6579:2002 was to be followed for the trial. Salmonella counts in each baby food were around 10 CFU/25 g (low) or 10 CFU/g (high level). Trial participants received five samples of each sample type and five blank samples. All samples contained Pseudomonas aeruginosa and Lactobacillus spp. at concentrations between 10(4) and 10(5) CFU/g as background flora. Precision data for the powdered infant formula were similar for the solid selective plating-out medium xylose-lysine-deoxycholate (XLD) and a 2nd choice of agar suitable to isolate Salmonella spp. The sensitivity was 91.3%, accordance 82.5% and concordance 83.9% for the low and 100% for the high level in all cases. For the cereal-based weaning food, the precision data for the high level were similar for XLD and the 2nd choice medium (98.8%, 97.5% and 97.5%). A slight difference was observed for the low level where XLD obtained values of 82.5%, 73.8%, 70.6% for sensitivity, accordance and concordance and the second choice medium values of 81.5%, 72.5% and 69%. The specificity, accordance and concordance of blanks for the infant formula and cereal-based weaning food were 100%.
Authors:
Renata G K Leuschner; Jan Bew; Martin P Boughtflower
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  International journal of food microbiology     Volume:  97     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-05     Completed Date:  2004-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  43-51     Citation Subset:  IM    
Affiliation:
Department for Environment, Food and Rural Affairs, Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK. r.leuschner@csl.gov.uk
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MeSH Terms
Descriptor/Qualifier:
Agar / chemistry*
Cereals / microbiology
Colony Count, Microbial / methods,  standards*
Food Analysis / standards*
Food Contamination / analysis
Food Microbiology
Humans
Infant
Infant Food / microbiology*
Infant Formula
Infant, Newborn
Laboratories / standards*
Reference Standards
Reproducibility of Results
Salmonella / isolation & purification*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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