Document Detail

A cold-sensitive mutation in 16S rRNA provides evidence for helical switching in ribosome assembly.
MedLine Citation:
PMID:  7681419     Owner:  NLM     Status:  MEDLINE    
A C-->U substitution at position 23 of 16S rRNA confers a dominant, cold-sensitive phenotype. The mutation changes the G11-C23 base pair of the 5' terminal pseudoknot helix to a G-U pair, which is predicted to cause significant weakening of the helix. Ribosomes containing mutant RNA are impaired in assembly and function at low temperature. Cells expressing the C23U mutation have decreased polysome levels and accumulate free 30S and 50S subunits and particles that resemble those previously observed in cold-sensitive alleles of ribosomal protein S5 and in in vitro reconstitution of 30S subunits carried out at low temperature. Three second-site suppressor mutations suggest that cold sensitivity is caused by competition between the 5' helix and an alternative helix formed by base-pairing of the upstream precursor sequence with one strand of the mature helix. Cold sensitivity appears to be relieved by destabilization of the competing precursor helix relative to the mature helix.
C S Dammel; H F Noller
Related Documents :
21991309 - Spatial genetic structure of a symbiotic beetle-fungal system: toward multi-taxa integr...
22026499 - A novel sgce gene mutation causing myoclonus dystonia in a family with an unusual pheno...
6322419 - Fine structure physical map locations of alterations that affect cell fusion in herpes ...
22194199 - Association between peroxisome proliferator-activated receptor gamma coactivator-1 alph...
21689189 - Latitudinal and voltinism compensation shape thermal reaction norms for growth rate.
8246899 - The vacuolar h(+)-atpase of saccharomyces cerevisiae is required for efficient copper d...
21072599 - Lack of gnaq germline mutations in uveal melanoma patients with high risk for hereditar...
23135349 - Genetic and epigenetic markers in the evaluation of pancreatic masses.
23314539 - A multiplex assay to rapidly exclude hla-dq2.5 and hla-dq8 expression in patients at ri...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  7     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-04-29     Completed Date:  1993-04-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  660-70     Citation Subset:  IM    
Sinsheimer Laboratories, University of California, Santa Cruz 95064.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Cold Temperature
Escherichia coli / genetics
Molecular Sequence Data
Nucleic Acid Conformation*
Point Mutation
RNA, Bacterial / analysis
RNA, Ribosomal, 16S / genetics*
Restriction Mapping
Ribosomal Proteins / genetics*
Ribosomes / metabolism*
Suppression, Genetic
Grant Support
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/Ribosomal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cytogenetic analysis of the paternal sex ratio chromosome of Nasonia vitripennis.
Next Document:  Purified MHC class I molecules present hapten-conjugated peptides to TNP/H-2Kb-specific T cell hybri...