| The clotting and staining of methylcellulose cultures of human hemopoietic cells. | |
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MedLine Citation:
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PMID: 6195007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a technique for clotting, fixing and staining methylcellulose cultures of human hemopoietic progenitor cells. The entire cellular population of a 1-ml culture in a 35-mm plastic petri dish can be recovered and preserved permanently with this method. The technique also provides the opportunity for distinguishing between pure erythroid and mixed colonies, for examining cellular morphology without tediously picking individual colonies, and for terminating cultures on the appropriate day without the necessity of scoring on that same day. |
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Authors:
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P Chan; S Shekhter-Levin; D Amato |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental hematology Volume: 11 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 1983 Sep |
Date Detail:
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Created Date: 1983-12-17 Completed Date: 1983-12-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 709-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Coagulation* Cells, Cultured Erythrocytes / cytology* Fixatives / diagnostic use Hematopoietic Stem Cells / classification, cytology* Histocytochemistry Humans Infant, Newborn Methylcellulose / pharmacology* Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Fixatives; 9004-67-5/Methylcellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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