| The cloning of plant E2F, a retinoblastoma-binding protein, reveals unique and conserved features with animal G(1)/S regulators. | |
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MedLine Citation:
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PMID: 10446243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Association of the retinoblastoma (Rb) protein with E2F transcription factors is central to cell cycle-specific gene expression and growth in animal cells. Whether Rb-E2F complexes are also involved in plant cell growth and differentiation is still unknown since E2F proteins have not yet been identified in plants. Here we report the isolation and characterisation of a wheat E2F (TmE2F) cDNA clone. Interestingly, the overall domain organisation of plant E2F is related to the human E2F-1/2/3 subset but its primary sequence is slightly more related to the E2F-4/5 subset. TmE2F-Rb binding depends on residues, located at the C-terminus, which are different from those of animal E2Fs. However, the acidic or hydrophobic nature of certain residues is maintained, strongly suggesting that they may have a crucial role in E2F activities. Plant E2F is expressed in proliferating cultured cells and in differentiated tissues and is up-regulated early in S phase. Our studies reinforce the idea that G(1)/S regulators in plants are unrelated to those of yeast cells but similar to those of animal cells and provide new tools to analyse the links between cell cycle regulators, plant growth and developmental signals. |
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Authors:
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E Ramírez-Parra; Q Xie; M B Boniotti; C Gutierrez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleic acids research Volume: 27 ISSN: 1362-4962 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-10-21 Completed Date: 1999-10-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3527-33 Citation Subset: IM |
Affiliation:
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Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AJ238590 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Carrier Proteins* Cell Cycle / physiology Cell Cycle Proteins* Cells, Cultured Cloning, Molecular Conserved Sequence DNA, Complementary / analysis DNA-Binding Proteins* E2F Transcription Factors E2F1 Transcription Factor E2F4 Transcription Factor Gene Library Genes, Plant* Humans Hydroxyurea / metabolism Molecular Sequence Data Protein Binding Recombinant Fusion Proteins / metabolism Retinoblastoma Protein / metabolism* Retinoblastoma-Binding Protein 1 Sequence Homology, Amino Acid Transcription Factor DP1 Transcription Factors / genetics* Triticum / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DNA, Complementary; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/E2F1 Transcription Factor; 0/E2F1 protein, human; 0/E2F4 Transcription Factor; 0/E2F4 protein, human; 0/Recombinant Fusion Proteins; 0/Retinoblastoma Protein; 0/Retinoblastoma-Binding Protein 1; 0/Transcription Factor DP1; 0/Transcription Factors; 127-07-1/Hydroxyurea |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 1 Month: 9 Year: 1999 Volume: 27 Issue: 17 First Page: 3527 Last Page: 3533 ID: 148597 PubMed Id: 10446243 Publisher Item Identifier: gkc527 |
| The cloning of plant E2F, a retinoblastoma-binding protein, reveals unique and conserved features with animal G(1)/S regulators. | |
| E Ram?rez-Parra | |
| Q Xie | |
| M B Boniotti | |
| C Gutierrez | |
| Centro de Biolog?a Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Cient?ficas, Universidad Aut?noma de Madrid, Cantoblanco, 28049 Madrid, Spain. |
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