Document Detail

A clinical comparison of the enzyme-linked immunosorbent assay (ELISA) and haemagglutination (TRC) in the routine detection of antithyroglobulin antibodies.
MedLine Citation:
PMID:  6344529     Owner:  NLM     Status:  MEDLINE    
The enzyme-linked immunosorbent assay (ELISA) has been proposed as an alternative to haemagglutination (TRC) in the routine examination of sera for autoantibodies such as thyroglobulin antibody (Anti-Tg). However, very few clinical comparisons of the two tests, incorporating matched controls and an independent standard, have so far been published. We have compared the performance of ELISA and TRC as screening procedures in 814 patients referred to our autoimmune profile laboratory with clinically suspected thyrotoxicosis, Hashimoto's thyroiditis or myxoedema. Radioimmunoassay (RIA) was used as the independent standard, and the normal range for each test was established from 100 carefully age and sex-matched controls. Sixty-five % of the sera were negative in all three tests. The results from ELISA and RIA were very similar (r = 0.88, P less than 0.001), with no major discrepancy. TRC, however, was negative in 101 (40.2%) of 251 RIA positive sera (r = 0.61, P less than 0.001) and in 118 (44.2%) or 267 ELISA positive patients (r = 0.69, P less than 0.001). Some of the TRC negative sera were strongly RIA and ELISA positive. ELISA is fully quantitative, cheap, and was found to be more accurate than TRC in identifying anti-Tg positive individuals. It has the potential to replace TRC in the routine screening of sera for anti-Tg antibodies.
D L Ewins; T J Wilkin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta endocrinologica     Volume:  103     ISSN:  0001-5598     ISO Abbreviation:  Acta Endocrinol.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-07-29     Completed Date:  1983-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370312     Medline TA:  Acta Endocrinol (Copenh)     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  216-22     Citation Subset:  IM    
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MeSH Terms
Antibodies / analysis*
Autoantibodies / analysis*
Enzyme-Linked Immunosorbent Assay*
Hemagglutination Tests*
Hyperthyroidism / immunology
Immunoenzyme Techniques*
Middle Aged
Myxedema / immunology
Thyroglobulin / immunology*
Thyroiditis, Autoimmune / immunology
Reg. No./Substance:
0/Antibodies; 0/Autoantibodies; 9010-34-8/Thyroglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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