| The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila. | |
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MedLine Citation:
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PMID: 19960024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cleaved-Caspase-3 antibody is a popular tool in apoptosis research in Drosophila. As the antibody was raised against cleaved human Caspase-3, it was assumed that it detects cleaved DRICE and DCP-1, Caspase-3-like effector caspases in Drosophila. However, as shown here, strong immunoreactivity persists in apoptotic models doubly mutant for drICE and dcp-1. In contrast, mutants of the apoptosome components DRONC (Caspase-9-like) and ARK (Apaf-1 related) do not label with the cleaved-Caspase-3 antibody. By peptide blocking experiments and further genetic studies, we provide evidence that the cleaved-Caspase-3 antibody recognizes multiple proteins including DCP-1 and likely DRICE, but also at least one additional unknown protein, all of which require DRONC for epitope exposure. The unknown substrate may be involved in non-apoptotic functions of DRONC. Because the cleaved-Caspase-3 antibody not only detects cleaved Caspase-3-like proteins in Drosophila, but also other proteins in a DRONC-dependent manner, it is more accurate to consider the cleaved-Caspase-3 antibody as a marker for DRONC activity, rather than effector caspase activity. |
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Authors:
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Y Fan; A Bergmann |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-12-04 |
Journal Detail:
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Title: Cell death and differentiation Volume: 17 ISSN: 1476-5403 ISO Abbreviation: Cell Death Differ. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-15 Completed Date: 2010-07-09 Revised Date: 2012-01-24 |
Medline Journal Info:
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Nlm Unique ID: 9437445 Medline TA: Cell Death Differ Country: England |
Other Details:
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Languages: eng Pagination: 534-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Graduate Program in Genes and Development, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard-Unit 1000, Houston, TX 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apoptosis / physiology Biological Markers / metabolism Caspase 3 / genetics, metabolism* Caspase 9 / genetics, metabolism* Caspases / genetics, metabolism* Drosophila Proteins / genetics, metabolism* Drosophila melanogaster / metabolism* Epitopes Humans Molecular Sequence Data Sequence Alignment |
| Grant Support | |
ID/Acronym/Agency:
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GM068016/GM/NIGMS NIH HHS; GM074977/GM/NIGMS NIH HHS; GM081543/GM/NIGMS NIH HHS; R01 GM068016-06/GM/NIGMS NIH HHS; R01 GM068016-08/GM/NIGMS NIH HHS; R01 GM068016-10/GM/NIGMS NIH HHS; R01 GM074977-03/GM/NIGMS NIH HHS; R01 GM081543-03/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Drosophila Proteins; 0/Epitopes; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases; EC 3.4.22.-/Dcp-1 protein, Drosophila; EC 3.4.22.-/Ice protein, Drosophila; EC 3.4.22.-/Nc protein, Drosophila |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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