Document Detail


A class V chitin synthase gene, chsA is essential for conidial and hyphal wall strength in the fungus Colletotrichum graminicola (Glomerella graminicola).
MedLine Citation:
PMID:  12684017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Colletotrichum graminicola tagged mutant T30 has conidia that burst and hyphal tips that swell in media with low osmotic pressure. The disrupted gene in T30 was identified as a class V chitin synthase (CSV) "chsA," which has an open reading frame of 1783 amino acids and two introns that are 52 and 54 bp. C. graminicola has one copy of chsA and no other highly homologous class V CHSs. Reverse transcriptase PCR indicated that the T30 mutant does not express the chsA transcript fragment in the conserved region in CSVs. Complementation of the mutant with chsA indicates that the encoded protein is responsible for approximately 29% of the chitin in conidial walls, is essential for conidial wall strength in media with high water potential and contributes to strength of hyphal tips. Analysis of the aligned deduced amino acid sequences of the 10 fully sequenced CSVs suggests that they are in two subgroups.
Authors:
Alongkorn Amnuaykanjanasin; Lynn Epstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  38     ISSN:  1087-1845     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-09     Completed Date:  2003-06-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  272-85     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, University of California, 1 Shields Avenue, Davis, CA 95616-8680, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY064208
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Chitin Synthase / chemistry,  genetics*,  metabolism
Colletotrichum / genetics,  growth & development*
Gene Dosage
Gene Expression Regulation, Fungal*
Genes, Essential*
Genetic Complementation Test
Molecular Sequence Data
Morphogenesis
Mutation
Plasmids
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
EC 2.4.1.16/Chitin Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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