Document Detail

A cis-acting tRNA gene imposes the cell cycle progression requirement for establishing silencing at the HMR locus in yeast.
MedLine Citation:
PMID:  21135074     Owner:  NLM     Status:  MEDLINE    
Numerous studies have determined that the establishment of Sir protein-dependent transcriptional silencing in yeast requires progression through the cell cycle. In our study we examined the cell cycle requirement for the establishment of silencing at the HML and HMR loci using strains bearing conditional or inducible SIR3 alleles. Consistent with prior reports, we observed that establishing silencing at HMR required progression through the cell cycle. Unexpectedly, we found that the HML locus is far less dependent on cell cycle progression to establish silencing. Seeking cis-acting elements that could account for this difference, we found that deletion of a tRNA gene that serves as a chromatin boundary at HMR abolishes the cell cycle progression requirement at this locus, while insertion of sequences containing this tRNA gene adjacent to HML imposes dependence on cell cycle progression for the full establishment of silencing. Our results indicate that the cell cycle progression requirement is not a property intrinsic to the formation of heterochromatin in yeast, but is instead a cis-limited, locus-specific phenomenon. We show that inactivation of the Scc1 cohesin also abolishes the requirement for cell cycle progression and test models based on a possible link between the tRNA gene and cohesin association.
Asmitha G Lazarus; Scott G Holmes
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-12-06
Journal Detail:
Title:  Genetics     Volume:  187     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-10     Completed Date:  2011-05-26     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  425-39     Citation Subset:  IM    
Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, Connecticut 06459, USA.
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MeSH Terms
Antineoplastic Agents / pharmacology
Cell Cycle / drug effects,  genetics*
Fungal Proteins / genetics,  metabolism
Gene Expression Regulation, Fungal
Gene Order
Gene Silencing*
RNA, Transfer / genetics*,  metabolism*
Yeasts / cytology,  genetics*,  metabolism*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Fungal Proteins; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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