| The circulating hormonal milieu of the endocrine pancreas in healthy individuals, organ donors, and the isolated perfused human pancreas. | |
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MedLine Citation:
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PMID: 10975715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although basal circulating levels of individual islet cell hormones have been measured, few studies compared the molar ratios of the major hormones secreted by the endocrine pancreas. This study examined the basal levels of four major islet hormones: insulin, C-peptide (C-P), glucagon (G), and pancreatic polypeptide (PP) in normal subjects, in organ donors with brain death, and in the isolated perfused human pancreas. Basal blood samples were taken from normal, fasted control subjects (NCs). Pancreata were obtained from 17 organ donors (ODs) with donor portal vein (DPV) and radial arterial (DRA) blood samples taken before organ procurement. Single-pass perfusion was performed on the procured pancreata, and after rewarming and equilibration, basal samples were collected from the splenic vein (SV) for 30 min. Radioimmunoassays of insulin, C-P, G, and PP were performed on all samples, and basal levels of all hormones were expressed as a common unit, femtomoles per milliliter. The data suggest that in the basal state, these four major islet hormones circulate in a relatively constant molar ratio. The ratio of the hormones is altered in brain death and with in vitro perfusion of the pancreas. The isolated perfused human pancreas secretes a relatively constant molar ratio of these hormones; however, this ratio is markedly different from the circulating ratio seen in either the NC group or the OD group. We conclude that a relatively constant hormonal milieu is secreted from the normal endocrine pancreas, and this hormonal milieu is altered after brain death and with isolation and perfusion of the human pancreas. |
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Authors:
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F C Brunicardi; Y Dyen; L Brostrom; R Kleinman; J Colonna; H Gelabert; R Gingerich |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Pancreas Volume: 21 ISSN: 0885-3177 ISO Abbreviation: Pancreas Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-12-06 Completed Date: 2000-12-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 203-11 Citation Subset: IM |
Affiliation:
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Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA. cbrunica@bcm.tmc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult C-Peptide / blood, secretion Female Glucagon / blood, secretion Hormones / blood*, secretion Humans Insulin / blood, secretion Islets of Langerhans / blood supply, secretion* Male Middle Aged Pancreatic Polypeptide / blood, secretion Perfusion Portal Vein Radial Artery Radioimmunoassay Splenic Vein Tissue Donors* |
| Grant Support | |
ID/Acronym/Agency:
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R29 DK 46441-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/C-Peptide; 0/Hormones; 11061-68-0/Insulin; 59763-91-6/Pancreatic Polypeptide; 9007-92-5/Glucagon |
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