Document Detail


A circadian rhythm regulating hyphal melanization in Cercospora kikuchii.
MedLine Citation:
PMID:  20943572     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many metabolic and developmental processes in fungi are controlled by biological rhythms. Circadian rhythms approximate a daily (24 h) cycle and have been thoroughly studied in the model fungus, Neurospora crassa. However relatively few examples of true circadian rhythms have been documented among other filamentous fungi. In this study we describe a circadian rhythm underlying hyphal melanization in Cercospora kikuchii, an important pathogen of soybean. After growth in light or light : dark cycles, colonies transferred to darkness produced zonate bands of melanized hyphae interspersed with bands of hyaline hyphae. Rhythmic production of bands was remarkably persistent in the absence of external cues, lasting at least 7 d after transfer to darkness, and was compensated over a range of temperatures. As in N. crassa, blue light but not red light was sufficient to entrain the circadian rhythm in C. kikuchii, and a putative ortholog of white collar-1, one of the genes required for light responses in N. crassa, was identified in C. kikuchii. Circadian regulation of melanization is conserved in other members of the genus: Similar rhythms were identified in another field isolate of C. kikuchii as well as field isolates of C. beticola and C. sorghi, but not in wild-type strains of C. zeae-maydis or C. zeina. This report represents the first documented circadian rhythm among Dothideomycete fungi and provides a new opportunity to dissect the molecular basis of circadian rhythms among filamentous fungi.
Authors:
Burton H Bluhm; A Michele Burnham; Larry D Dunkle
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-07-13
Journal Detail:
Title:  Mycologia     Volume:  102     ISSN:  0027-5514     ISO Abbreviation:  Mycologia     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-10-15     Completed Date:  2010-12-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0400764     Medline TA:  Mycologia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1221-8     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, University of Arkansas Division of Agriculture, Fayetteville, Arkansas 72701, USA.
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MeSH Terms
Descriptor/Qualifier:
Ascomycota / physiology*,  radiation effects*
Circadian Rhythm / radiation effects*
Hyphae / physiology,  radiation effects
Light

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