Document Detail


A chronic scheme of cranial window preparation to study pial vascular reactivity in murine cerebral malaria.
MedLine Citation:
PMID:  23279271     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The acute implantation of a cranial window for studying cerebroarteriolar reactivity in living animals involves a highly surgically invasive craniotomy procedure at the time of experimentation, which limits its application in severely ill animals such as in the experimental murine model of cerebral malaria (ECM). To overcome this problem, a chronic window implantation scheme was designed and implemented.
METHODS: A partial craniotomy is first performed by creating a skull bone flap in the healthy mice, which are then left to recover for one to two weeks, followed by infection to induce ECM. Uninfected animals are utilized as control. When cranial superfusion is needed, the bone flap is retracted and window implantation completed by assembling a perfusion chamber for compound delivery to the exposed brain surface. The presurgical step is intended to minimize surgical trauma on the day of experimentation.
RESULTS: Chronic preparations in uninfected mice exhibited remarkably improved stability over acute ones by significantly reducing periarteriolar tissue damage and enhancing cerebroarteriolar dilator responses. The chronic scheme was successfully implemented in ECM mice, which unveiled novel preliminary insights into impaired cerebroarteriolar reactivity and eNOS dysfunction.
CONCLUSION: The chronic scheme presents an innovative approach for advancing our mechanistic understanding on cerebrovascular dysfunction in ECM.
Authors:
Peng Kai Ong; Diana Meays; John A Frangos; Leonardo J M Carvalho
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  20     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-11     Completed Date:  2014-02-04     Revised Date:  2014-07-02    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  394-404     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrovascular Circulation*
Malaria, Cerebral / pathology,  physiopathology*
Mice
Nitric Oxide Synthase Type III / metabolism
Plasmodium berghei*
Skull / surgery*
Grant Support
ID/Acronym/Agency:
R01 AI082610/AI/NIAID NIH HHS; R01 HL087290/HL/NHLBI NIH HHS; R01-AI82610/AI/NIAID NIH HHS; R01-HL87290/HL/NHLBI NIH HHS; R37 HL040696/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, mouse
Comments/Corrections

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