Document Detail

A chronic pressure ulcer model in the nude mouse.
MedLine Citation:
PMID:  19614912     Owner:  NLM     Status:  MEDLINE    
The pathophysiology of pressure ulcers is not yet fully understood. Widely used experimental models lead to pressure ulcers in the skin and the subcutaneous tissue only. The aim of the present study was to develop a clinically relevant deep pressure ulcer model in nude mice involving all relevant tissue layers. Balb/c nude mice were anesthetized and a steel disk was implanted under the great gluteus muscle. Full recovery was allowed for 10 days. Pressure was then periodically applied (2 hours compression, 1 hour recovery) using a Neodymium magnet in conjunction with the disk in the unanesthetized mouse. Cycles were repeated for 1, 4, 6, 8, or 10 times. Controls underwent the same procedure without placement of the magnet. The pressure ulcer grade was found to be cycle dependent,with the highest degree after 10 cycles. Histological evaluations revealed signs of necrosis in the skin and subcutaneous fat after four, and in the muscle after eight and 10 cycles of compression. Polymorphnuclear granulocyte infiltration in certain layers was found to be dependent on the number of cycles. We conclude that this clinically relevant nude mouse model can be used to investigate the mechanism of pressure ulcer development and to study new therapeutic approaches.
Esther Wassermann; Martijn van Griensven; Karin Gstaltner; Wolfgang Oehlinger; Karl Schrei; Heinz Redl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  17     ISSN:  1524-475X     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  480-4     Citation Subset:  IM    
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, 1200 Vienna, Austria.
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MeSH Terms
Disease Models, Animal*
Mice, Nude
Pressure Ulcer / pathology*,  physiopathology*

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