Document Detail


A chromosomal Borrelia burgdorferi gene encodes a 22-kilodalton lipoprotein, P22, that is serologically recognized in Lyme disease.
MedLine Citation:
PMID:  8027338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe the isolation of the gene encoding a 22-kDa antigen from Borrelia burgdorferi, the etiologic agent of Lyme disease. The p22 gene is 582 nucleotides in length and encodes a protein of 194 amino acids with a predicted molecular mass of 21.8 kDa. The leader signal sequence of P22 consists of a positively charged short amino terminus, a central hydrophobic domain, and at the carboxyl terminus, a cleavage site that is presumably recognized and cleaved by a B. burgdorferi signal peptidase. P22 has 98.5% homology with the recently described B. burgdorferi protein IpLA7. P22 is processed as a lipoprotein, as demonstrated by [3H]palmitate labeling. Pulsed-field gel electrophoresis showed that p22, like LA7, is localized to the linear chromosome of B. burgdorferi. Examination of sera from patients with Lyme disease revealed that antibodies to P22 are rarely detected in patients with early-stage disease characterized by erythema migrans (2 of 20), and 35% of the patients with late-stage disease characterized by arthritis (9 of 26) developed antibodies to P22. Sera from patients with syphilis did not react with P22. When patients with late-stage disease were tested for their antibody reactivities to four other outer surface proteins (OspA), OspB, OspE, and OspF), 75% of these patients responded to P22 or to one or more outer surface proteins.
Authors:
T T Lam; T P Nguyen; E Fikrig; R A Flavell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  32     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-08-05     Completed Date:  1994-08-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  876-83     Citation Subset:  IM    
Affiliation:
Section of Immunobiology, Howard Hughes Medical Institute, New Haven, Connecticut.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L22530;  X70826
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Bacterial / blood
Antigens, Bacterial / chemistry,  genetics
Bacterial Proteins / chemistry,  genetics*,  immunology*
Base Sequence
Binding Sites / genetics
Borrelia burgdorferi Group / genetics*,  immunology*
Chromosome Mapping
Cloning, Molecular
DNA, Bacterial / genetics
Genes, Bacterial*
Humans
Lipoproteins / chemistry,  genetics,  immunology
Lyme Disease / diagnosis,  immunology,  microbiology*
Molecular Sequence Data
Molecular Weight
Rabbits
Grant Support
ID/Acronym/Agency:
AI30548/AI/NIAID NIH HHS; U5-CCU-106581//PHS HHS
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/Lipoproteins
Comments/Corrections

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