Document Detail


A chromogenic medium for the detection of yeasts with beta-galactosidase and beta-glucosidase activities from intermediate moisture foods.
MedLine Citation:
PMID:  10826724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A selective and differential solid medium for the specific detection of some common yeasts frequently causing spoilage in intermediate moisture foods is described. The principle of the method is based on the detection of two enzymes, beta-glucosidase and beta-galactosidase, using the chromogenic substrates salmon-Gluc and X-Gal. Over 140 yeasts and bacteria were tested, and Debaryomyces hansenii and Kluyveromyces marxianus strains produced salmon and dark blue colonies, respectively, thus permitting their clear discrimination from other yeasts common in intermediate moisture foods. The medium was very satisfactory when intermediate moisture foods were tested.
Authors:
M I de Silóniz; M J Valderrama; J M Peinado
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  63     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-08-01     Completed Date:  2000-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  651-4     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología, Facultad de Biología, Universidad Complutense de Madrid, Spain. siloniz@eucmax.sim.ucm.es
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MeSH Terms
Descriptor/Qualifier:
Chromogenic Compounds*
Culture Media
Food Microbiology*
Kluyveromyces / enzymology
Microbiological Techniques
Saccharomycetales / enzymology
Yeasts / enzymology*
beta-Galactosidase / metabolism*
beta-Glucosidase / metabolism*
Chemical
Reg. No./Substance:
0/Chromogenic Compounds; 0/Culture Media; EC 3.2.1.21/beta-Glucosidase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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