| The chromic chloride method of coupling antigens to erythrocytes: definition of some important parameters. | |
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MedLine Citation:
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PMID: 1254974 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Factors affecting the efficiency of the chronic cloride method of coupling antigen to erythrocytes were studied using a radioactive tracer. The amount of chromic chloride required for optimal coupling was related to the concentration of protein in the reaction mixture. Coupling was considerably more efficient when the chromic chloride solution was allowed to 'age' prior to use. In the system studied, the addition of piperazine buffer made little difference to the degree of coupling. The reaction was shown to proceed very rapidly. 50% of the final uptake of antigen took place within seconds, and the reaction was essentially complete 5 min after initiation. The importance of adequate mixing during the addition of the chromic chloride solution was emphasised. |
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Authors:
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J W Goding |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunological methods Volume: 10 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1976 |
Date Detail:
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Created Date: 1976-05-18 Completed Date: 1976-05-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 61-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens* Binding Sites, Antibody Chlorides / metabolism Chromium* Erythrocytes / immunology* Sheep Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Binding Sites, Antibody; 0/Chlorides; 7440-47-3/Chromium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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