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A chromatospectroscopic method for analyzing the signals generated during high-performance liquid chromatography with diode-array detection.
MedLine Citation:
PMID:  11318068     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A "chromatospectroscopic" technique has been developed to quantitate two compounds that coelute in high-performance liquid chromatography. The method uses a diode-array detector with Millenium 32 software to extract spectra at regular time intervals during the elution of the unique peak and recover spectral data (absorbance versus wavelength), which can then be processed using the Excel software package. The method is applied to mixtures of two coeluting UV filters. Both could be accurately quantitated even when the mixture consisted of 99.5% of one and only 0.5% of the other.
Authors:
J D Fourneron
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  39     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-24     Completed Date:  2001-07-12     Revised Date:  2003-10-31    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  160-4     Citation Subset:  -    
Affiliation:
Laboratoire de Chimie Analytique de l'Environnement, Faculté des Sciences et Techniques de Saint-Jér me, Marseille, France.
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