Document Detail


The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle.
MedLine Citation:
PMID:  18064355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G1 and S phases as well as at the G1/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G1 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Authors:
Katrin Zimmermann; Marlis Holtz; Loc Phi-van
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-21
Journal Detail:
Title:  Biological research     Volume:  40     ISSN:  0716-9760     ISO Abbreviation:  Biol. Res.     Publication Date:  2007  
Date Detail:
Created Date:  2007-12-07     Completed Date:  2008-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9308271     Medline TA:  Biol Res     Country:  Chile    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
Affiliation:
Institute for Animal Welfare and Animal Husbandry, Federal Agricultural Research Centre, Dörnbergstr. 25-27, 29223 Celle, Germany.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / genetics*,  physiology
Cell Line
Chromatin / chemistry,  genetics*,  metabolism
DNA Replication / genetics*
Humans
Transcription Factors / genetics*
Chemical
Reg. No./Substance:
0/Chromatin; 0/Transcription Factors; 0/YEATS4 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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