Document Detail


On-chip single-cell microcultivation assay for monitoring environmental effects on isolated cells.
MedLine Citation:
PMID:  12763026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a on-chip single-cell microcultivation assay as a means of observing the adaptation process of single bacterial cells during nutrient concentration changes. This assay enables the direct observation of single cells captured in microchambers made on thin glass slides and having semipermeable membrane lids, in which cells were kept isolated with optical tweezers. After changing a medium of 0.2% (w/v) glucose concentration to make it nutrient-free 0.9% NaCl medium, the growth of all cells inserted into the medium stopped within 20 min, irrespective of their cell cycles. When a nutrient-rich medium was restored, the cells started to grow again, even after the medium had remained nutrient-free for 42 h. The results indicate that the cell's growth and division are directly related to their nutrient condition. The growth curve also indicates that the cells keep their memory of what their growth and division had been before they stopped growing.
Authors:
Senkei Umehara; Yuichi Wakamoto; Ippei Inoue; Kenji Yasuda
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  305     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-23     Completed Date:  2003-07-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  534-40     Citation Subset:  IM    
Affiliation:
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Bacteriological Techniques / instrumentation,  methods*
Cell Division
Culture Media
Environment
Escherichia coli / growth & development*,  isolation & purification,  physiology
Kinetics
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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