Document Detail


On-chip magnetic separation and encapsulation of cells in droplets.
MedLine Citation:
PMID:  23370785     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Single cell study is gaining importance because of the cell-to-cell variation that exists within cell population, even after significant initial sorting. Analysis of such variation at the gene expression level could impact single cell functional genomics, cancer, stem-cell research, and drug screening. The on-chip monitoring of individual cells in an isolated environment would prevent cross-contamination, provide high recovery yield, and enable study of biological traits at a single cell level. These advantages of on-chip biological experiments is a significant improvement for a myriad of cell analyses methods, compared to conventional methods, which require bulk samples and provide only averaged information on cell structure and function. We report on a device that integrates a mobile magnetic trap array with microfluidic technology to provide the possibility of separation of immunomagnetically labeled cells and their encapsulation with reagents into picoliter droplets for single cell analysis. The simultaneous reagent delivery and compartmentalization of the cells immediately following sorting are all performed seamlessly within the same chip. These steps offer unique advantages such as the ability to capture cell traits as originated from its native environment, reduced chance of contamination, minimal use of the reagents, and tunable encapsulation characteristics independent of the input flow. Preliminary assay on cell viability demonstrates the potential for the device to be integrated with other up- or downstream on-chip modules to become a powerful single-cell analysis tool.
Authors:
Aaron Chen; Tom Byvank; Woo-Jin Chang; Atul Bharde; Greg Vieira; Brandon L Miller; Jeffrey J Chalmers; Rashid Bashir; Ratnasingham Sooryakumar
Related Documents :
23689165 - Intracellular atp-binding cassette transporter a3 is expressed in lung cancer cells and...
22929415 - Vitrification of in vitro produced bovine embryos: effect of embryonic block and develo...
23026135 - Geminin functions downstream of p53 in k-ras-induced gene amplification of dihydrofolat...
23788285 - Low-power laser irradiation promotes the proliferation and osteogenic differentiation o...
16525765 - Methods for fabrication of nanoscale topography for tissue engineering scaffolds.
9434785 - Telomerase activity in human leukemic cell lines is inhibited by antisense pentadecadeo...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-1
Journal Detail:
Title:  Lab on a chip     Volume:  -     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Physics, The Ohio State University, Columbus, Ohio, 43210, USA. soory@mps.ohio-state.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Taking personality selection bias seriously in animal cognition research: a case study in capuchin m...
Next Document:  DFT comparison of the OH-initiated degradation mechanisms for five chlorophenoxy herbicides.