Document Detail

On-chip magnetic separation and encapsulation of cells in droplets.
MedLine Citation:
PMID:  23370785     Owner:  NLM     Status:  MEDLINE    
Single cell study is gaining importance because of the cell-to-cell variation that exists within cell population, even after significant initial sorting. Analysis of such variation at the gene expression level could impact single cell functional genomics, cancer, stem-cell research, and drug screening. The on-chip monitoring of individual cells in an isolated environment would prevent cross-contamination, provide high recovery yield, and enable study of biological traits at a single cell level. These advantages of on-chip biological experiments is a significant improvement for a myriad of cell analyses methods, compared to conventional methods, which require bulk samples and provide only averaged information on cell structure and function. We report on a device that integrates a mobile magnetic trap array with microfluidic technology to provide the possibility of separation of immunomagnetically labeled cells and their encapsulation with reagents into picoliter droplets for single cell analysis. The simultaneous reagent delivery and compartmentalization of the cells immediately following sorting are all performed seamlessly within the same chip. These steps offer unique advantages such as the ability to capture cell traits as originated from its native environment, reduced chance of contamination, minimal use of the reagents, and tunable encapsulation characteristics independent of the input flow. Preliminary assay on cell viability demonstrates the potential for the device to be integrated with other up- or downstream on-chip modules to become a powerful single-cell analysis tool.
Aaron Chen; Tom Byvank; Woo-Jin Chang; Atul Bharde; Greg Vieira; Brandon L Miller; Jeffrey J Chalmers; Rashid Bashir; Ratnasingham Sooryakumar
Related Documents :
23354575 - Synchrotron ftir microspectroscopy of escherichia coli at single-cell scale under silve...
23947765 - Non-small cell lung cancer cells survived ionizing radiation treatment display cancer s...
24256735 - Memory and modularity in cell-fate decision making.
23724115 - Increased cell proliferation and mucocyte density in the sea anemone aiptasia pallida r...
8578995 - Replication of a mamestra brassicae nuclear polyhedrosis virus in a newly established m...
23354575 - Synchrotron ftir microspectroscopy of escherichia coli at single-cell scale under silve...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-20     Completed Date:  2013-08-27     Revised Date:  2014-10-12    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1172-81     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line, Tumor
Cell Separation
Cell Survival
Microfluidic Analytical Techniques / instrumentation,  methods*
Mineral Oil / chemistry
Single-Cell Analysis
Grant Support
Reg. No./Substance:
8020-83-5/Mineral Oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Taking personality selection bias seriously in animal cognition research: a case study in capuchin m...
Next Document:  DFT comparison of the OH-initiated degradation mechanisms for five chlorophenoxy herbicides.