| On-chip epithelial barrier function assays using electrical impedance spectroscopy. | |
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MedLine Citation:
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PMID: 20379587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A bio-impedance chip has been developed for real-time monitoring of the kinetics of epithelial cell monolayers in vitro. The human bronchial epithelial cell line (16-HBE 14o-) was cultured in Transwells creating a sustainable and interactive model of the airway epithelium. Conducting polymer polypyrrole (PPy) doped with polystyrene sulfonate (PSS) was electrochemically deposited onto the surface of gold-plated electrodes to reduce the influence of the electrical double layer on the impedance measurements. Finite element and equivalent circuit models were used to model and determine the electrical properties of the epithelial cell monolayer from the impedance spectra. Electrically tight, confluent monolayers of 16 HBE 14o- cells were treated with increasing concentrations of either Triton X-100 to solubilize cell membranes or ethylene glycol-bis(2-aminoethyl-ether)-N,N,N'N'-tetraacetic acid (EGTA) to disrupt cell-cell adhesion. Experimental impedance data showed that disruption of epithelial barrier function in response to Triton X-100 and EGTA can be successfully measured by the bio-impedance chip. The results were consistent with the conventional hand-held trans-epithelial electrical resistance measurements. Immunofluorescent staining of the ZO-1 tight junction protein in the untreated and treated 16HBEs was performed to verify the disruption of the tight junctions by EGTA. |
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Authors:
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Tao Sun; Emily J Swindle; Jane E Collins; Judith A Holloway; Donna E Davies; Hywel Morgan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-08 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-02 Completed Date: 2010-07-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 1611-7 Citation Subset: IM |
Affiliation:
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Nano Research Group, School of Electronics and Computer Science, University of Southampton, SO17 1BJ, United Kingdom. ts5@ecs.soton.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Electric Impedance Electrodes Epithelial Cells / cytology* Humans Kinetics Microchip Analytical Procedures* Polymers / chemistry Polystyrenes / chemistry Pyrroles / chemistry Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Polymers; 0/Polystyrenes; 0/Pyrroles; 28210-41-5/polystyrene sulfonic acid; 30604-81-0/polypyrrole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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