Document Detail

On-chip connector valve for immunoaffinity chromatography in a microfluidic chip.
MedLine Citation:
PMID:  17623428     Owner:  NLM     Status:  MEDLINE    
A miniature valve that operates between a chip port and a tube fitting was developed. The valve functions by means of a rotor, 3 mm in diameter and 1.5 mm in height, made of Teflon, with a 0.2-mm diameter hole at its center that is co-axial with the tube fitting. It also has a radial groove, 0.85 mm long, 0.2 mm wide, and 0.2 mm deep, at the bottom surface, starting at its center. The chip port and the tube fitting have an offset of 0.75 mm, and, thus, the rotation of the rotor can make an on and off connection between the chip port and the groove, which is connected to the tubing. The valve had a pressure resistance of at least 1.0 MPa. The on-chip valve can be placed in position by adding only a single part, a valve rotor, and no changes in the fabrication of the glass microchip are required. Since the valve functions as a part of a connector, we refer to it as an on-chip connector valve. Immunoaffinity chromatography of a fluorescence-labeled recombinant antibody fragment was carried out in a glass microchip using the valves.
Kiyohito Shimura; Yutaka Koyama; Kae Sato; Takehiko Kitamori
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of separation science     Volume:  30     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-11     Completed Date:  2007-10-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1477-81     Citation Subset:  IM    
Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
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MeSH Terms
Chromatography, Affinity* / instrumentation,  methods
Equipment Design
Fluorescent Dyes / chemistry
Immunoglobulin Fragments / chemistry
Immunologic Techniques* / instrumentation
Materials Testing
Microfluidic Analytical Techniques* / instrumentation,  methods
Recombinant Proteins / chemistry
Reg. No./Substance:
0/Fluorescent Dyes; 0/Immunoglobulin Fragments; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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