Document Detail


On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes.
MedLine Citation:
PMID:  18214684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The metabolic activity of cells can be monitored by measuring the pH in the extracellular environment. Microfabrication and microfluidic technologies allow the sensor size and the extracellular volumes to be comparable to single cells. A glass substrate with thin film pH sensitive IrO( x ) electrodes was sealed to a replica-molded polydimethylsiloxane (PDMS) microfluidic network with integrated valves. The device, termed NanoPhysiometer, allows the trapping of single cardiac myocytes and the measurement of the pH in a detection volume of 0.36 nL. For wild-type (WT) single cardiac myocytes an acidification rate of 6.45 +/- 0.38 mpH/min was measured in comparison to 19.5 +/- 0.38 mpH/min for very long chain Acyl-CoA dehydrogenase (VLCAD) deficient mice in 0.8 mM of Ca(2+). VLCAD deficiency is a fatty acid oxidation disease leading to cardiomyopathy and arrhythmias. The acidification rate increased to 11.96 +/- 1.33 mpH/min for WT and to 32.0 +/- 4.64 mpH/min for VLCAD -/- in 1.8 mM of Ca(2+). The NanoPhysiometer concept can be extended to study ischemia/reperfusion injury or disorders of other biological systems to identify strategies for treatment and possible pharmacological targets.
Authors:
Igor A Ges; Igor A Dzhura; Franz J Baudenbacher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical microdevices     Volume:  10     ISSN:  1387-2176     ISO Abbreviation:  Biomed Microdevices     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  United States    
Other Details:
Languages:  eng     Pagination:  347-54     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / instrumentation,  methods
Cell Separation / instrumentation*,  methods
Cells, Cultured
Electrochemistry / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
Hydrogen-Ion Concentration*
Ion-Selective Electrodes*
Mice
Microchemistry / instrumentation,  methods
Microfluidic Analytical Techniques / instrumentation*,  methods
Myocytes, Cardiac / chemistry*,  metabolism
Nanotechnology / instrumentation*,  methods
Grant Support
ID/Acronym/Agency:
5U01AI061223-03/AI/NIAID NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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