Document Detail


A chimera of EBNA1 and the estrogen receptor activates transcription but not replication.
MedLine Citation:
PMID:  1738208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA replication from the plasmid origin of replication of Epstein-Barr virus requires one viral protein, EBNA1. This protein also acts as a transcriptional activator. Mutational analyses of EBNA1 have led to the conclusion that it supports transcription and DNA replication similarly. Such analyses have not probed the DNA-binding domain of EBNA1. To test whether domains of EBNA1 specifically required for either transcription or replication lie within its DNA-binding domain, we constructed a functional transcriptional activator by placing the EBNA1 DNA-binding domain in the context of the activation domains of the estrogen receptor. This hybrid protein did not support DNA replication, which indicates that the DNA-binding domain does not contain a replication-specific domain that can function along with heterologous transcriptional activating domains.
Authors:
T Middleton; B Sugden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-03-16     Completed Date:  1992-03-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1795-8     Citation Subset:  IM    
Affiliation:
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antigens, Viral / genetics*
Base Sequence
DNA Replication
DNA-Binding Proteins / metabolism*
Epstein-Barr Virus Nuclear Antigens
Gene Expression Regulation, Viral
Humans
Molecular Sequence Data
Receptors, Estrogen / genetics*
Recombinant Fusion Proteins / metabolism
Regulatory Sequences, Nucleic Acid
Structure-Activity Relationship
Transcription, Genetic
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA-07175/CA/NCI NIH HHS; CA-22443/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Viral; 0/DNA-Binding Proteins; 0/Epstein-Barr Virus Nuclear Antigens; 0/Receptors, Estrogen; 0/Recombinant Fusion Proteins
Comments/Corrections

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