Document Detail

A chemical relaxation study of human prostatic acid phosphatase.
MedLine Citation:
PMID:  5641399     Owner:  NLM     Status:  MEDLINE    
Chemical relaxation methods and a dilution technique were applied to the study of the hydrolysis of p-nitrophenyl phosphate by human prostatic acid phosphatase. Although the reaction mechanism was not elucidated, rate constants and equilibrium constants were obtained for the reaction of enzyme and p-nitrophenol to form a complex. A slow, 2-sec relaxation effect which showed no concentration dependence was observed in various reaction mixtures, including some lacking the substrate and products of the hydrolytic reaction. The conclusion drawn is that there are two forms of the prostatic enzyme, which are normally in equilibrium with each other, but which undergo a relatively slow interconversion when this equilibrium is perturbed. A preliminary calculation indicates that these forms are present in the equilibrium ratio of 2:1.
D B Shear; K Kustin
Related Documents :
35719 - Testicular acid phosphatases of the mouse. characterization after chromatographic fract...
17375469 - Cytochemical localization of acid phosphatase in stigeoclonium tenue (chaetophorales, c...
110149 - The baboon as a primate model for the study of endometrium.
563889 - The cytochemical demonstration of gerl in rat hepatocytes during lipoprotein mobilization.
2162959 - Enzyme cytochemistry of the alveolar sacs and golgian-like cisternae in the ciliate ich...
1608029 - Ultrastructural localization of alkaline and acid phosphatase activities in dental plaque.
6164259 - Effect of various sources of organic carbon and high nitrite and nitrate concentrations...
7383979 - A simple method for the preparation of d-alpha-aminoadipic acid.
15035369 - Mycotoxin-forming ability of two penicillium roqueforti strains in blue moldy tulum che...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical journal     Volume:  8     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1968 Jan 
Date Detail:
Created Date:  1968-05-09     Completed Date:  1968-05-09     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  139-43     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acid Phosphatase / metabolism*
Progesterone / analysis
Prostate / enzymology*
Reg. No./Substance:
0/Isoenzymes; 57-83-0/Progesterone; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  X-ray diffraction study in water of lipids extracted from human erythrocytes: the position of choles...
Next Document:  A model of the peripheral auditory system responding to low-frequency tones.