Document Detail


The chemical modification of E. coli L-asparaginase by N,O-carboxymethyl chitosan.
MedLine Citation:
PMID:  8922226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
E. coli L-asparaginase was modified with N,O-carboxymethyl chitosan in the presence of normal product L-aspartic acid, which protected the active site of the enzyme. The modified enzyme remained high catalytic activity, showed greater stability against trypsin and alpha-chymotrypsin, but lost its activity more rapidly at high temperature (> 45 degrees C) than did the native enzyme. When tested in vivo, the plasma half-life of the modified enzyme (t1/2 = 40 hr) was over 33 times longer than that of the native enzyme (t1/2 = 1.6 hr). The results showed that the modified L-asparaginase may be much more useful than did the native enzyme for clinical treatments of tumors.
Authors:
G Qian; J Zhou; J Ma; D Wang; B He
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial cells, blood substitutes, and immobilization biotechnology     Volume:  24     ISSN:  1073-1199     ISO Abbreviation:  Artif Cells Blood Substit Immobil Biotechnol     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-03-04     Completed Date:  1997-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9431307     Medline TA:  Artif Cells Blood Substit Immobil Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  567-77     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Functional Polymers for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / chemistry,  pharmacokinetics*
Asparaginase / chemistry,  metabolism,  pharmacokinetics*
Chitin / analogs & derivatives*,  chemistry,  pharmacokinetics
Chitosan*
Drug Stability
Enzyme Stability
Escherichia coli / enzymology
Half-Life
Metabolic Clearance Rate
Rabbits
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 107043-88-9/O,N-carboxymethylchitosan; 1398-61-4/Chitin; 9012-76-4/Chitosan; EC 3.5.1.1/Asparaginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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