| A chemical approach to solving bridging phenomena in steroid radioimmunoassays. | |
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MedLine Citation:
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PMID: 7303028 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Steroid radioimmunoassays (RIA) employ antibodies raised against a carrier protein-steroid conjugate. Individual antibodies may recognize the steroid, the protein or the chemical bridge used to join them together. Use of the same bridge in the tracer results in higher affinity binding of the tracer than the native ligand which in turn results in a loss of sensitivity and precision. We have greatly reduced bridge-binding in a RIA for androstenedione. Conjugates and radioiodinated labels were prepared with either an ester or either chemical bridge. By using an antibody and the corresponding label with the heterologous bridge very sensitive assays were obtained. |
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Authors:
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G D Nordblom; R Webb; R E Counsell; B G England |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Steroids Volume: 38 ISSN: 0039-128X ISO Abbreviation: Steroids Publication Date: 1981 Aug |
Date Detail:
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Created Date: 1982-01-20 Completed Date: 1982-01-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0404536 Medline TA: Steroids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 161-73 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Androstenedione
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analysis* Animals Chemical Phenomena Chemistry False Positive Reactions Female Haptens Radioimmunoassay / methods* Sheep / immunology |
| Chemical | |
Reg. No./Substance:
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0/Haptens; 63-05-8/Androstenedione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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