Document Detail

A chemical approach to solving bridging phenomena in steroid radioimmunoassays.
MedLine Citation:
PMID:  7303028     Owner:  NLM     Status:  MEDLINE    
Steroid radioimmunoassays (RIA) employ antibodies raised against a carrier protein-steroid conjugate. Individual antibodies may recognize the steroid, the protein or the chemical bridge used to join them together. Use of the same bridge in the tracer results in higher affinity binding of the tracer than the native ligand which in turn results in a loss of sensitivity and precision. We have greatly reduced bridge-binding in a RIA for androstenedione. Conjugates and radioiodinated labels were prepared with either an ester or either chemical bridge. By using an antibody and the corresponding label with the heterologous bridge very sensitive assays were obtained.
G D Nordblom; R Webb; R E Counsell; B G England
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Steroids     Volume:  38     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1981 Aug 
Date Detail:
Created Date:  1982-01-20     Completed Date:  1982-01-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  161-73     Citation Subset:  IM    
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MeSH Terms
Androstenedione / analysis*
Chemical Phenomena
False Positive Reactions
Radioimmunoassay / methods*
Sheep / immunology
Reg. No./Substance:
0/Haptens; 63-05-8/Androstenedione

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