| A characterization and targeting of the infarct border zone in a Swine model of myocardial infarction. | |
| | |
MedLine Citation:
|
PMID: 23067355 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Introduction: Novel therapies for myocardial infarction (MI) involving stem cells, gene therapy, biomaterials, or revascularization strategies have shown promise in animal studies and clinical trials, but results have been limited partially due to the injection of therapeutics into ischemic myocardium that cannot support their mechanism of action. Accurate targeting of therapeutics precisely to the infarct border zone (BZ) may be essential for effective repair of the ischemic heart. Methods: Ischemia-reperfusion MI was induced in Yorkshire swine by inflation of an angioplasty balloon in the left anterior descending coronary artery. Fluorescent microspheres were injected into the BZ under NOGA catheter guidance, and this location was identified grossly then examined by immunohistochemistry and Western analysis. Results: Analysis of the infarct zone two hours post-MI revealed a frankly necrotic region devoid of contractile proteins with marked activation of caspase-3. The NOGA-defined BZ closely approximates the grossly-defined BZ and contains intact myocytes and vasculature. Western analysis detected Akt expression and levels of Ca(2+) handling proteins equivalent to that of viable tissues. Conclusions: Histological and Western analysis revealed that NOGA mapping precisely identifies grossly and molecularly defined infarct BZ at a location where there are still viable cells and vessels capable of supporting novel therapeutic strategies. Clin Trans Sci 2012; Volume 5: 416-421. |
| | |
Authors:
|
Jason M Duran; Sharven Taghavi; Remus M Berretta; Catherine A Makarewich; Thomas Sharp Iii; Tim Starosta; Foram Udeshi; Jon C George; Hajime Kubo; Steven R Houser |
Related Documents
:
|
2871485 - Silent ischemia as a marker for early unfavorable outcomes in patients with unstable an... 9327705 - Frequency, duration, magnitude, and consequences of myocardial ischemia during intracor... 1505565 - Clinical significance of silent ischaemia and implications for treatment: role of radio... 3871095 - Noninvasive testing in the evaluation of myocardial ischemia: agreement among tests. 10654505 - Myocardial tactile stiffness during acute reduction of coronary blood flow. 1273555 - Mitral valve replacement with ball and tilting disc valve prosthesis. a clinical and ha... |
Publication Detail:
|
Type: Journal Article Date: 2012-06-26 |
Journal Detail:
|
Title: Clinical and translational science Volume: 5 ISSN: 1752-8062 ISO Abbreviation: Clin Transl Sci Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101474067 Medline TA: Clin Transl Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 416-21 Citation Subset: IM |
Copyright Information:
|
© 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Temple University School of Medicine, Cardiovascular Research Center, Temple University. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Translation to practice of an intervention to promote colorectal cancer screening among african amer...
Next Document: Impedance of Novel Therapeutic Technologies: The Case of Stem Cells.