Document Detail


A characterization and targeting of the infarct border zone in a swine model of myocardial infarction.
MedLine Citation:
PMID:  23067355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Novel therapies for myocardial infarction (MI) involving stem cells, gene therapy, biomaterials, or revascularization strategies have shown promise in animal studies and clinical trials, but results have been limited partially due to the injection of therapeutics into ischemic myocardium that cannot support their mechanism of action. Accurate targeting of therapeutics precisely to the infarct border zone (BZ) may be essential for effective repair of the ischemic heart.
METHODS: Ischemia-reperfusion MI was induced in Yorkshire swine by inflation of an angioplasty balloon in the left anterior descending coronary artery. Fluorescent microspheres were injected into the BZ under NOGA catheter guidance, and this location was identified grossly then examined by immunohistochemistry and Western analysis.
RESULTS: Analysis of the infarct zone two hours post-MI revealed a frankly necrotic region devoid of contractile proteins with marked activation of caspase-3. The NOGA-defined BZ closely approximates the grossly-defined BZ and contains intact myocytes and vasculature. Western analysis detected Akt expression and levels of Ca(2+) handling proteins equivalent to that of viable tissues.
CONCLUSIONS: Histological and Western analysis revealed that NOGA mapping precisely identifies grossly and molecularly defined infarct BZ at a location where there are still viable cells and vessels capable of supporting novel therapeutic strategies.
Authors:
Jason M Duran; Sharven Taghavi; Remus M Berretta; Catherine A Makarewich; Thomas Sharp Iii; Tim Starosta; Foram Udeshi; Jon C George; Hajime Kubo; Steven R Houser
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Publication Detail:
Type:  Journal Article     Date:  2012-06-26
Journal Detail:
Title:  Clinical and translational science     Volume:  5     ISSN:  1752-8062     ISO Abbreviation:  Clin Transl Sci     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-16     Completed Date:  2013-09-04     Revised Date:  2014-01-16    
Medline Journal Info:
Nlm Unique ID:  101474067     Medline TA:  Clin Transl Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  416-21     Citation Subset:  IM    
Copyright Information:
© 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanical Phenomena
Blotting, Western
Disease Models, Animal*
Echocardiography
Fluorescent Antibody Technique
Heart Function Tests
Myocardial Infarction / metabolism,  pathology*,  physiopathology,  ultrasonography
Phosphoserine / metabolism
Sus scrofa
Grant Support
ID/Acronym/Agency:
P01 HL091799/HL/NHLBI NIH HHS; P01 HL108806/HL/NHLBI NIH HHS; R01 HL089312/HL/NHLBI NIH HHS; R37 HL033921/HL/NHLBI NIH HHS; T32 HL091804/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
17885-08-4/Phosphoserine
Comments/Corrections

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