Document Detail

The characterization of cell death induced by 1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl) cytosine (ECyd) in FM3A cells.
MedLine Citation:
PMID:  9672699     Owner:  NLM     Status:  MEDLINE    
The characterization of cell death induced by 1-(3-C-ethynyl-beta-D- ribopentofuranosyl) cytosine (ECyd), a potent inhibitor of RNA synthesis, was performed using mouse mammary tumor FM3A cells in vitro. Accompanied with the cell death induced by ECyd (3.0 muM) -treatment, about 100-200 kbp-sized and internucleosomal DNA fragmentation were observed by orthogonal-field-alternation gel electrophoresis (OFAGE) and conventional gel electrophoresis, respectively. Protease inhibitors, carbobenzoxy-L-aspart-1-yl[(2,6-dichlorobenzyl)oxy]methane (Z-Asp-CH2-DCB), N alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) and N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), effectively blocked the cell death, suggesting that the proteases inhibited by Z-Asp-CH2-DCB, TLCK or TPCK were involved in the process of cell death.
S Takatori; S Tsutsumi; M Hidaka; H Kanda; A Matsuda; M Fukushima; Y Wataya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleosides & nucleotides     Volume:  17     ISSN:  0732-8311     ISO Abbreviation:  Nucleosides Nucleotides     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-08-06     Completed Date:  1998-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8215930     Medline TA:  Nucleosides Nucleotides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1309-17     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Cell Death / drug effects
Cytidine / analogs & derivatives*,  pharmacology
DNA Damage / drug effects
Mammary Neoplasms, Experimental / pathology*
Nucleic Acid Synthesis Inhibitors / pharmacology*
Serine Proteinase Inhibitors / pharmacology
Tosyllysine Chloromethyl Ketone / pharmacology
Tosylphenylalanyl Chloromethyl Ketone / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/1-(3-C-ethynylribopentofuranosyl)cytosine; 0/Antineoplastic Agents; 0/Nucleic Acid Synthesis Inhibitors; 0/Serine Proteinase Inhibitors; 2104-86-1/Tosyllysine Chloromethyl Ketone; 402-71-1/Tosylphenylalanyl Chloromethyl Ketone; 65-46-3/Cytidine

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