Document Detail


The characteristics of elastic fiber assembled with recombinant tropoelastin isoform.
MedLine Citation:
PMID:  16690047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: It is known that elastin mRNA is transcribed from a single gene. The variety of tropoelastin isoforms results from multiple alternative splicing of the primary transcript. The purpose of this study was to investigate the characteristics of elastic fiber assembled with tropoelastin isoform, which is full-length human tropoelastin (HTE), exon 26A missing tropoelastin (Delta26A), and exon 32 missing tropoelastin (Delta32). DESIGN AND METHODS: We demonstrated the process of elastic fiber assembly and the existence of elastic fiber resistant to pancreatic elastase with HTE, Delta26A, or Delta32 fiber using an in vitro model of elastic fiber assembly. These elastic fibers were evaluated by immunofluorescent staining, the quantitative analysis of cross-linked amino acids, and semi-quantitative analysis of matrix-associated tropoelastin. RESULTS: There were no big differences getting into the matrix among these tropoelastins in immunofluorescence microscopy and semi-quantitative analysis. In the comparison with the HTE, the Delta26A and the Delta32 significantly increased and decreased, respectively, the formation of cross-linking amino acids and the binding to scaffold proteins. Furthermore, it was found that it is difficult to degrade the Delta26A assembly with pancreatic elastase as compared with HTE or Delta32 assembly. CONCLUSION: The elastic fiber assembled with the tropoelastin isoforms was characterized using an in vitro model. The present study provides important information regarding the pathology of human diseases including emphysema and atherosclerosis.
Authors:
Fumiaki Sato; Hiroshi Wachi; Barry C Starcher; Hayato Murata; Satoshi Amano; Shingo Tajima; Yoshiyuki Seyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-11
Journal Detail:
Title:  Clinical biochemistry     Volume:  39     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-17     Completed Date:  2006-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  746-53     Citation Subset:  IM    
Affiliation:
Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Science, 2-4-41 Ebara, Tokyo, 142-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Amino Acids / chemistry
Cross-Linking Reagents / chemistry
Exons
Fluorescent Antibody Technique
Humans
Microfilament Proteins / genetics,  metabolism
Pancreatic Elastase / metabolism
Protein Isoforms
Recombinant Proteins / genetics,  metabolism
Tropoelastin / chemistry,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cross-Linking Reagents; 0/Microfilament Proteins; 0/Protein Isoforms; 0/Recombinant Proteins; 0/Tropoelastin; 0/fibrillin; EC 3.4.21.36/Pancreatic Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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