Document Detail


The changes in the neuronal PC12 and the intestinal epithelial Caco-2 cells during the coculture. The functional analysis using an in vitro coculture system.
MedLine Citation:
PMID:  19003283     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The interaction between intestinal epithelial cells andperipheral neuronal cells were examined using an invitro coculture system. Two cell lines, Caco-2 and PC12, were usedfor this experiment as an intestinal epithelial and entericneuronal cell model, respectively. By coculturing with fullydifferentiated Caco-2 cells, the neurite outgrowth was inducedin PC12 cells. This neurite outgrowth in PC12 was blocked byanti-nerve growth factor (NGF) polyclonal antibodies,suggesting that the neurite outgrowth in PC12 during thecoculture with Caco-2 cells was due to NGF secreted fromCaco-2 cells. On the other hand, coculturing with fullydifferentiated PC12 cells induced the decrease oftransepithelial electrical resistance in Caco-2 cellmonolayers. The permeability of lucifer yellow alsosignificantly increased, suggesting that the barrier functionand paracellular permeability of Caco-2 monolayers werealtered by coculturing with PC12 cells. The present studysuggests that this in vitro coculture system is a good modelfor the functional analysis of interaction among intestinalepithelial cells with different cell types.
Authors:
H Satsu; T Yokoyama; N Ogawa; Y Fujiwara-Hatano; M Shimizu
Related Documents :
15269243 - Protective properties of neoechinulin a against sin-1-induced neuronal cell death.
11978393 - Inhibition of neurite outgrowth by reduced level of ndrg4 protein in antisense transfec...
24240163 - A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  35     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-03-05    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-9     Citation Subset:  -    
Affiliation:
Department of Applied Biological Chemistry, Graduate school of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hydroxyapatite-pulp composite fiber sheet bed: A new material for the immobilization of CHO-K1 cells...
Next Document:  Tissue specific expression and differential regulation by 1alpha,25-dihydroxyvitamin D3 of the calci...