| The changes in the neuronal PC12 and the intestinal epithelial Caco-2 cells during the coculture. The functional analysis using an in vitro coculture system. | |
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MedLine Citation:
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PMID: 19003283 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The interaction between intestinal epithelial cells andperipheral neuronal cells were examined using an invitro coculture system. Two cell lines, Caco-2 and PC12, were usedfor this experiment as an intestinal epithelial and entericneuronal cell model, respectively. By coculturing with fullydifferentiated Caco-2 cells, the neurite outgrowth was inducedin PC12 cells. This neurite outgrowth in PC12 was blocked byanti-nerve growth factor (NGF) polyclonal antibodies,suggesting that the neurite outgrowth in PC12 during thecoculture with Caco-2 cells was due to NGF secreted fromCaco-2 cells. On the other hand, coculturing with fullydifferentiated PC12 cells induced the decrease oftransepithelial electrical resistance in Caco-2 cellmonolayers. The permeability of lucifer yellow alsosignificantly increased, suggesting that the barrier functionand paracellular permeability of Caco-2 monolayers werealtered by coculturing with PC12 cells. The present studysuggests that this in vitro coculture system is a good modelfor the functional analysis of interaction among intestinalepithelial cells with different cell types. |
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Authors:
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H Satsu; T Yokoyama; N Ogawa; Y Fujiwara-Hatano; M Shimizu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 35 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2008-11-12 Completed Date: 2011-07-14 Revised Date: 2013-03-05 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 73-9 Citation Subset: - |
Affiliation:
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Department of Applied Biological Chemistry, Graduate school of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. |
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