Document Detail


The cellular prion protein with a monoacylated glycosylphosphatidylinositol anchor modifies cell membranes, inhibits cell signaling and reduces prion formation.
MedLine Citation:
PMID:  21738009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prion diseases occur following the conversion of the cellular prion protein (PrPC) into a disease-related isoform (PrPSc). In this study a cell painting technique was used to examine the role of the glycosylphosphatidylinositol (GPI) anchor attached to PrPC in prion formation. The introduction of PrPC to infected neuronal cells increased the cholesterol content of cell membranes, increased activation of cytoplasmic phospholipase A2 (cPLA2) and increased PrPSc formation. In contrast, PrPC with a monoacylated GPI anchor did not alter the amount of cholesterol in cell membranes, was not found within lipid rafts and did not activate cPLA2. Although monoacylated PrPC remains within cells for longer than native PrPC it was not converted to PrPSc. Moreover, the presence of monoacylated PrPC displaced cPLA2 from PrPSc-containing lipid rafts, reducing the activation of cPLA2 and PrPSc formation. We conclude that acylation of the GPI anchor attached to PrPC modifies the local membrane microenvironments that control some cell signaling pathways, the trafficking of PrPC and PrPSc formation. In addition, such observations raise the possibility that the pharmacological modification of GPI anchors might constitute a novel therapeutic approach to prion diseases.
Authors:
Clive Bate; Alun Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-01
Journal Detail:
Title:  Prion     Volume:  5     ISSN:  1933-690X     ISO Abbreviation:  Prion     Publication Date:    2011 Apr-Jun
Date Detail:
Created Date:  2011-08-08     Completed Date:  2012-01-27     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101472305     Medline TA:  Prion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  65-8     Citation Subset:  IM    
Affiliation:
Department of Pathology and Infectious Diseases, Royal Veterinary College, North Mymms, Herts, UK. on protein (PrP
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / metabolism
Cholesterol / metabolism
Glycosylphosphatidylinositols / metabolism*
Membrane Microdomains / metabolism*
Mice
Phospholipases A2 / metabolism
PrPC Proteins / metabolism
PrPSc Proteins / metabolism
Prions / metabolism*
Signal Transduction
Chemical
Reg. No./Substance:
0/Glycosylphosphatidylinositols; 0/PrPC Proteins; 0/PrPSc Proteins; 0/Prions; 57-88-5/Cholesterol; EC 3.1.1.4/Phospholipases A2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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