| A cellular isolation system for real-time single-cell oxygen consumption monitoring. | |
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MedLine Citation:
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PMID: 18522927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of a cellular isolation system (CIS) that enables the monitoring of single-cell oxygen consumption rates in real time is presented. The CIS was developed through a multidisciplinary effort within the Microscale Life Sciences Center (MLSC) at the University of Washington. The system comprises arrays of microwells containing Pt-porphyrin-embedded polystyrene microspheres as the reporter chemistry, a lid actuator system and a gated intensified imaging camera, all mounted on a temperature-stabilized confocal microscope platform. Oxygen consumption determination experiments were performed on RAW264.7 mouse macrophage cells as proof of principle. Repeatable and consistent measurements indicate that the oxygen measurements did not adversely affect the physiological state of the cells measured. The observation of physiological rates in real time allows studies of cell-to-cell heterogeneity in oxygen consumption rate to be performed. Such studies have implications in understanding the role of mitochondrial function in the progression of inflammatory-based diseases, and in diagnosing and treating such diseases. |
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Authors:
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Joe Dragavon; Tim Molter; Cody Young; Tim Strovas; Sarah McQuaide; Mark Holl; Meng Zhang; Brad Cookson; Alex Jen; Mary Lidstrom; Deirdre Meldrum; Lloyd Burgess |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the Royal Society, Interface / the Royal Society Volume: 5 Suppl 2 ISSN: 1742-5689 ISO Abbreviation: J R Soc Interface Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-08-21 Completed Date: 2008-10-30 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 101217269 Medline TA: J R Soc Interface Country: England |
Other Details:
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Languages: eng Pagination: S151-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Washington, Seattle, WA 98195-2180, USA. joedragavon@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calibration Cell Respiration Cell Separation / instrumentation*, standards Cells, Cultured Cytological Techniques / instrumentation Mice Oxygen Consumption* Reproducibility of Results |
| Grant Support | |
ID/Acronym/Agency:
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P50 HG 002360/HG/NHGRI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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