Document Detail


The cell wall of the Arabidopsis pollen tube--spatial distribution, recycling, and network formation of polysaccharides.
MedLine Citation:
PMID:  23037507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pollen tube is a cellular protuberance formed by the pollen grain, or male gametophyte, in flowering plants. Its principal metabolic activity is the synthesis and assembly of cell wall material, which must be precisely coordinated to sustain the characteristic rapid growth rate and to ensure geometrically correct and efficient cellular morphogenesis. Unlike other model species, the cell wall of the Arabidopsis (Arabidopsis thaliana) pollen tube has not been described in detail. We used immunohistochemistry and quantitative image analysis to provide a detailed profile of the spatial distribution of the major cell wall polymers composing the Arabidopsis pollen tube cell wall. Comparison with predictions made by a mechanical model for pollen tube growth revealed the importance of pectin deesterification in determining the cell diameter. Scanning electron microscopy demonstrated that cellulose microfibrils are oriented in near longitudinal orientation in the Arabidopsis pollen tube cell wall, consistent with a linear arrangement of cellulose synthase CESA6 in the plasma membrane. The cellulose label was also found inside cytoplasmic vesicles and might originate from an early activation of cellulose synthases prior to their insertion into the plasma membrane or from recycling of short cellulose polymers by endocytosis. A series of strategic enzymatic treatments also suggests that pectins, cellulose, and callose are highly cross linked to each other.
Authors:
Youssef Chebli; Minako Kaneda; Rabah Zerzour; Anja Geitmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-04
Journal Detail:
Title:  Plant physiology     Volume:  160     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-16     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1940-55     Citation Subset:  IM    
Affiliation:
Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montreal, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology*,  ultrastructure
Biomechanics
Cell Wall / metabolism*,  ultrastructure
Cellulose / metabolism
Esterification
Fucose / metabolism
Glucans / metabolism
Microfibrils / metabolism
Microscopy, Fluorescence
Models, Biological
Pectins / metabolism
Pollen Tube / cytology*,  ultrastructure
Polysaccharides / metabolism*
Pyridinium Compounds / metabolism
Quaternary Ammonium Compounds / metabolism
Xylans / metabolism
Chemical
Reg. No./Substance:
0/FM 4-64; 0/Glucans; 0/Pectins; 0/Polysaccharides; 0/Pyridinium Compounds; 0/Quaternary Ammonium Compounds; 0/Xylans; 3713-31-3/Fucose; 37294-28-3/xyloglucan; 9004-34-6/Cellulose; 9064-51-1/callose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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