| The cell wall of the Arabidopsis thaliana pollen tube - spatial distribution, recycling and network formation of polysaccharides. | |
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MedLine Citation:
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PMID: 23037507 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The pollen tube is a cellular protuberance formed by the pollen grain, or male gametophyte, in flowering plants. Its principal metabolic activity is the synthesis and assembly of cell wall material which must be precisely coordinated to sustain the characteristic rapid growth rate and to ensure geometrically correct and efficient cellular morphogenesis. Unlike other model species, the cell wall of the Arabidopsis thaliana pollen tube has not been described in detail. We used immuno-histochemistry and quantitative image analysis to provide a detailed profile of the spatial distribution of the major cell wall polymers composing the Arabidopsis pollen tube cell wall. Comparison with predictions made by a mechanical model for pollen tube growth revealed the importance of pectin de-esterification in determining the cell diameter. Scanning electron microscopy demonstrated that cellulose microfibrils are oriented in near longitudinal orientation in the Arabidopsis pollen tube cell wall, consistent with a linear arrangement of cellulose synthase CESA6 in the plasma membrane. Cellulose label was also found inside cytoplasmic vesicles and might originate from an early activation of cellulose synthases prior to their insertion into the plasma membrane or from recycling of short cellulose polymers by endocytosis. A series of strategic enzymatic treatments also suggests that pectins, cellulose and callose are highly cross-linked to each other. |
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Authors:
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Youssef Chebli; Minako Kaneda; Rabah Zerzour; Anja Geitmann |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-4 |
Journal Detail:
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Title: Plant physiology Volume: - ISSN: 1532-2548 ISO Abbreviation: Plant Physiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Montreal. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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