Document Detail


A cell surface-displayed anti-c-myc single-chain antibody: new perspectives for the genetic improvement of cellular tumor vaccines.
MedLine Citation:
PMID:  11023198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have shown recently that cell surface-bound, single-chain Fv antibodies (scFv) are a powerful tool for the improvement of cellular tumor vaccines. To simplify this approach and to develop a general tool for the generation and improvement of cellular tumor vaccines, we chose an scFv against a peptide from the human proto-oncogene c-myc that could anchor any c-myc-tagged protein to the cell surface. The retroviral vector p50-Mx-neo (pMESV) was used to express scFv on the surface of the human melanoma line SkMel63. The cell-bound anti-c-myc scFv bound specifically to a soluble purified anti-CD28 scFv carrying a c-myc peptide-tag at its C terminus. Proof of principle was determined by incubating human peripheral blood lymphocytes with a mixture of (a) anti-c-myc-transfected SkMel63 cells binding the anti-CD28 scFv and (b) SkMel63 cells transfected with an anti-CD3 scFv. A clear synergistic effect on T-cell activation was observed that was comparable with that obtained in previous studies using SkMel63 cells transfected with the gene for the anti-CD28 scFv. As the cell surface-displayed anti-c-myc scFv can bind any c-myc-tagged protein of interest, this technique facilitates the genetic engineering of cellular vaccines for the therapy of virtually all human neoplasias.
Authors:
C de Inés; B Cochlovius; S Schmidt; M Little
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer gene therapy     Volume:  7     ISSN:  0929-1903     ISO Abbreviation:  Cancer Gene Ther.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-10     Completed Date:  2001-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9432230     Medline TA:  Cancer Gene Ther     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1257-62     Citation Subset:  IM    
Affiliation:
Recombinant Antibody Group, Experimental Therapy and Diagnosis Programme, German Cancer Research Center, Heidelberg.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology*
Antigens, Surface / immunology*
Binding Sites
Cancer Vaccines / therapeutic use*
Cell Division / drug effects
DNA Primers / chemistry
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression
Genes, myc / genetics,  immunology*
Genetic Vectors
Humans
Immunogenetics
Immunoglobulin Fragments / immunology*
Interferon-gamma / analysis
Interleukin-4 / analysis
Lymphocyte Activation / immunology*
Polymerase Chain Reaction
T-Lymphocytes / immunology*
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Surface; 0/Cancer Vaccines; 0/DNA Primers; 0/Immunoglobulin Fragments; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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