Document Detail

A cell surface desmosome-associated component: identification of tissue-specific cell adhesion molecule.
MedLine Citation:
PMID:  3532108     Owner:  NLM     Status:  MEDLINE    
Autoantibodies in the serum of patients suffering the blistering skin disease pemphigus vulgaris recognize a 140-kDa glycoprotein (GP) present in enriched fractions of bovine tongue epidermal desmosomes. Immunofluorescence observations of cryostat sections of bovine tongue epidermis reveal that affinity-purified rabbit antibodies to the 140-kDa GP generate a punctate intercellular stain that is similar to that generated by antibodies directed against a desmosome plaque component (desmoplakin). In cultured mouse keratinocytes, the antibodies against 140-kDa GP recognize desmosomes along areas of cell-cell contact. Double immunofluorescence of cultured keratinocytes with these antibodies and a desmoplakin antiserum reveals that the antibodies against the 140-kDa GP stain a single fluorescent line along areas of cell-cell contact. This single fluorescent line lies between double fluorescent lines generated by the desmoplakin antiserum. Immunogold ultrastructural localization reveals that the 140-kDa antigen is localized not only along the intercellular area of the desmosome but also is found along the whole epidermal cell surface. The antibodies to the 140-kDa GP are able to induce a disruption of cell-cell contact in cultured keratinocytes that possess desmosomes. We propose that the 140-kDa GP is a cell adhesion molecule (CAM). Furthermore we discuss the heterogeneity of desmosomes in the light of our findings that antibodies against the 140-kDa GP recognize specific stratified squamous epithelial tissues.
J C Jones; K M Yokoo; R D Goldman
Related Documents :
11140368 - Detailed study of the different taurine uptake systems of colon lovo mdr and non-mdr ce...
7986198 - Comparison of mechanisms responsible for resistance to idarubicin and daunorubicin in m...
9051118 - Analysis of dna content in multidrug-resistant cells by image and flow cytometry.
17943188 - The multidrug transporter p-glycoprotein: a mediator of melanoma invasion?
22034098 - Plasmodium induced by su6656, an src family kinase inhibitor, is accompanied by a contr...
24901228 - Targeting echinococcus multilocularis stem cells by inhibition of the polo-like kinase ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-10-30     Completed Date:  1986-10-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7282-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, Surface / immunology*
Autoantibodies / isolation & purification
Cell Adhesion*
Desmosomes / immunology*
Epidermis / immunology,  ultrastructure
Extracellular Space / ultrastructure
Fluorescent Antibody Technique
Microscopy, Electron
Molecular Weight
Pemphigus / immunology
Reg. No./Substance:
0/Antigens, Surface; 0/Autoantibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial ce...
Next Document:  Intranuclear appearance of the phosphorylated form of cytoskeleton-associated 350-kDa proteins in U1...