| The cell surface of Aeromonas salmonicida determines in vitro survival in cultured brook trout (Salvelinus fontinalis) peritoneal macrophages. | |
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MedLine Citation:
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PMID: 8971685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aeromonas salmonicida strains phenotypically differing in their A-layer, lipopolysaccharide, and macrophage cytotoxicity were tested in vitro for survivability in brook trout (Salvelinus fontinalis) serum with or without antibodies, and in vivo following intraperitoneal injection. The ability of brook trout peritoneal macrophages to phagocytize and kill the different phenotypes was investigated in an in vitro assay. The virulent strain, A. salmonicida 80204, which has the full complement of known virulence factors, as well as the recently described macrophage cytotoxin, was resistant in vitro to both the bactericidal activity of normal and immune serum, and to brook trout peritoneal macrophages. A. salmonicida SS-70.1, which possesses the A-layer but lacks the cytotoxin, was resistant to the bactericidal activity of normal and immune serum but was avirulent and killed by macrophages. Phenotypes lacking the A-layer, regardless of whether or not they possessed the macrophage cytotoxin were avirulent, susceptible to normal and immune serum and the bactericidal activity of peritoneal macrophages. A. salmonicida virulence expression requires both the A-layer and the macrophage cytotoxin. |
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Authors:
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J G Daly; A K Kew; A R Moore; G Olivier |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microbial pathogenesis Volume: 21 ISSN: 0882-4010 ISO Abbreviation: Microb. Pathog. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-03-18 Completed Date: 1997-03-18 Revised Date: 2006-09-23 |
Medline Journal Info:
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Nlm Unique ID: 8606191 Medline TA: Microb Pathog Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 447-61 Citation Subset: IM |
Affiliation:
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Halifax Fisheries Research Laboratory, Department of Fisheries and Oceans, NS, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aeromonas
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immunology,
metabolism*,
pathogenicity* Animals Antibodies, Bacterial / immunology Cells, Cultured Cytotoxicity, Immunologic Cytotoxins / immunology, metabolism* Fishes / blood, immunology, microbiology* Gram-Negative Bacterial Infections / immunology* Immunization Lipopolysaccharides / analysis*, immunology, metabolism* Macrophages, Peritoneal / immunology, microbiology* Phagocytosis / immunology* Proteins* Staphylococcal Protein A / immunology, metabolism* Virulence |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Cytotoxins; 0/Lipopolysaccharides; 0/Proteins; 0/Staphylococcal Protein A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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